CUSTOM ITEM: HPLC Column, RP C18, Wide Pore, 10um, 4.0mm ID x 250mm Length, 300A. Cogent Brand. 1 EA.
Additional Info:
The 68018‑25P‑4‑3M Cogent RP C18 Wide Pore HPLC Column is designed for maximum separation performance in biomolecule method development and preparative scouting, combining 10 µm particles with a 300 Å wide‑pore structure to deliver enhanced analyte accessibility, improved recovery, and reliable chromatographic performance.
Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding and fully double end‑capped, this stationary phase provides:
Reduced silanol interactions
Improved peak shape for proteins, peptides, and large polar compounds
Consistent and reproducible chromatography
The 300 Å pore size enables large molecules to fully access the stationary phase, minimizing restricted diffusion and improving recovery compared to standard 100 Å media.
This column is ideal as a USP L1 alternate for large molecule applications and as a method development platform for preparative scale‑up.
250 mm Length – Maximum Separation & Peak Capacity
Highest resolving power in the 4.0 mm wide‑pore C18 range
Maximizes peak capacity for highly complex biomolecule mixtures
Ideal for proteins, peptides, and closely related macromolecules
Provides extended retention for large and difficult analytes
Best suited for final method optimization prior to preparative scale‑up
This format is selected when shorter columns cannot achieve required resolution or separation performance.
4.0 mm ID – Solvent‑Saving Analytical Format
Comparable chromatographic performance to 4.6 mm ID columns
Reduces mobile phase consumption and waste
Typical flow rate range: ~0.8–1.2 mL/min
Optimal starting range: ~0.9–1.1 mL/min
Benefits:
Lower solvent consumption
Reduced operating cost
Compatible with standard HPLC systems
Flow Rate & System Considerations (4.0 mm ID, 10 µm, 300 Å)
Suitable for standard HPLC systems
Supports higher flow rates and increased sample loading
Well suited for:
Preparative method development
Biomolecule scouting workflows
Analytical‑to‑preparative scale‑up
Why Choose Wide Pore (300 Å) C18 (10 µm)
Designed for Large Molecules
Enables separation of:
Proteins
Peptides
Large biomolecules
Eliminates mass transfer limitations of 100 Å stationary phases
Ideal for Preparative Scouting & Scale‑Up
Used to develop and refine methods prior to preparative chromatography
Predictable selectivity when transitioning to larger ID preparative columns
Supports efficient scale‑up workflows
Maintains Reversed‑Phase Selectivity
Retains classic C18 hydrophobic interaction behavior
Suitable for lipids and long‑chain fatty acids
Cogent RP C18™ Advantages
Fully End‑Capped Surface
Minimizes secondary interactions
Improves peak symmetry
Better performance for basic analytes
High Purity Silica
Consistent chromatographic performance
Reduced variability between runs
Durable & Cost‑Efficient
Long column lifetime
Ideal for repeated method development cycles
Reduced maintenance requirements
Typical Applications
Preparative biomolecule method development
Protein and peptide separations
Complex biomolecule mixtures
Hydrophobic and neutral compound separations
Long‑chain fatty acids
Analytical to preparative scale‑up workflows
USP L1 wide‑pore reversed‑phase methods
Important Usage Notes
Designed for large molecule separations using 300 Å pore media
Compatible with standard HPLC systems
Ideal for maximum‑resolution separations prior to preparative scale‑up
Choose when loading capacity and robustness are prioritized over efficiency
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable