Catalog Number - 68018-25P-4

The 68018‑25P‑4 Cogent RP C18 HPLC Column is designed for maximum separation performance in method development and preparative scouting, utilizing 10 µm particles and fully double end‑capped C18 bonding to deliver reliable retention, durability, and consistent chromatographic performance.

Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding, this stationary phase provides:

• Complete end‑capping to minimize silanol interactions

• Improved peak symmetry for polar and basic analytes

• Consistent and reproducible chromatography

This column is ideal as a USP L1 alternate and as a method development platform for scaling analytical methods to preparative separations.

250 mm Length – Maximum Separation & Peak Capacity

• Highest resolving power in the 4.0 mm RP C18 range

• Maximizes peak capacity for highly complex mixtures

• Ideal for challenging separations and closely related analytes

• Provides extended retention for hydrophobic compounds

• Best suited for final method optimization prior to preparative scale‑up

This format is selected when shorter columns cannot achieve required resolution or separation performance.

4.0 mm ID – Solvent‑Saving Analytical Format

• Comparable chromatographic behavior to 4.6 mm ID columns

• Reduces mobile phase consumption and waste

• Typical flow rate range: ~0.8–1.2 mL/min

• Optimal starting range: ~0.9–1.1 mL/min

Benefits:

• Lower solvent usage

• Reduced operating cost

  Compatible with standard HPLC systems

Flow Rate & System Considerations (4.0 mm ID, 10 µm)

• Suitable for standard HPLC systems

• Supports higher flow rates and increased sample loading

• Well suited for:

  • Preparative method development

  • High‑loading analytical scouting

  • Scale‑up method optimization

Why Choose RP C18 (10 µm)

Ideal for Preparative Scouting & Scale‑Up

• Used to develop and refine methods prior to preparative chromatography

• Predictable selectivity when transitioning to larger ID preparative columns

• Supports efficient analytical‑to‑preparative workflows

Reliable Hydrophobic Retention

• Strong reversed‑phase retention for hydrophobic and neutral compounds

• Excellent for long‑chain fatty acids

• Provides greater retention than C8 phases

Method Development & USP Compatibility

• Suitable for USP L1 methods

• Ideal for new analytical and preparative method development

• Reliable and consistent stationary phase chemistry

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry

• Enhances performance for basic analytes

High Purity Silica

• Consistent chromatographic performance

• Reduced variability between runs

Durable & Cost‑Efficient

• Long column lifetime

• Ideal for repeated method development cycles

• Lower maintenance requirements

Typical Applications

• Preparative method development and scouting

• Complex mixture separations

• Hydrophobic and neutral compound analysis

• Long‑chain fatty acids

• Analytical to preparative scale‑up workflows

• USP L1 reversed‑phase methods

Important Usage Notes

• Designed for method development and preparative scaling workflows

• Compatible with standard HPLC systems

• Ideal for maximum‑resolution separations prior to scale‑up

• Choose when loading capacity and robustness are prioritized over efficiency

Made‑to‑Order & Pricing Information

This is a custom, made‑to‑order item.

• Orders cannot be canceled once placed

• Custom items are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification:

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.