Catalog Number - 68018-25P-3M

The 68018‑25P‑3M Cogent RP C18 Wide Pore HPLC Column is engineered for maximum separation performance of large molecular weight analytes, combining 10 µm particles with a 300 Å wide‑pore structure to deliver enhanced analyte accessibility, improved recovery, and consistent chromatographic results.

Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding and fully double end‑capped, this stationary phase provides:

• Reduced silanol interactions

• Improved peak shape for proteins, peptides, and macromolecules

• Reliable and reproducible chromatography

The 300 Å pore size allows large biomolecules to fully access the stationary phase, minimizing restricted diffusion and improving efficiency compared to standard 100 Å columns.

This column is ideal as a USP L1 alternate for large molecule methods, and is especially useful for legacy and regulatory methods requiring wide‑pore separations.

250 mm Length – Maximum Separation & Peak Capacity

• Highest resolving power in the 4.6 mm wide‑pore C18 range

• Maximizes peak capacity for complex biomolecule mixtures

• Ideal for proteins, peptides, and closely related macromolecules

• Provides extended retention for large analytes

• Best suited for final method validation and high‑purity separations

This configuration is selected when shorter columns cannot achieve the required resolution or separation performance.

4.6 mm ID – Standard Analytical Performance

• Industry‑standard HPLC column dimension

• Compatible with conventional analytical systems

• Typical flow rate range: ~0.8–1.5 mL/min

• Optimal starting range: ~1.0–1.2 mL/min

Provides:

• Strong detector response

• High reproducibility

• Easy method transfer across laboratories

Flow Rate & System Considerations (4.6 mm ID, 10 µm, 300 Å)

• Suitable for standard HPLC systems

• Supports higher flow rates and increased sample loading

• Well suited for:

  • Biomolecule method development

  • Preparative scouting workflows

  • Legacy method replication and validation

Why Choose Wide Pore (300 Å) C18 (10 µm)

Designed for Large Molecules

• Enables efficient separation of:

  • Proteins

  • Peptides

  • Large biomolecules

• Eliminates mass transfer limitations of 100 Å stationary phases

Ideal for Preparative Scouting & Scale‑Up

• Used to develop and refine methods prior to preparative chromatography

• Predictable selectivity when transitioning to larger preparative columns

• Supports efficient analytical‑to‑preparative workflows

Maintains Reversed‑Phase Selectivity

• Retains classic C18 hydrophobic interaction behavior

• Suitable for lipids and long‑chain fatty acids

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry

• Enhances performance for basic analytes

High Purity Silica

• Consistent chromatographic results

• Reduced variability between runs

Durable & Cost‑Efficient

• Long column lifetime

• Ideal for validated and repeated workflows

• Lower maintenance frequency

Typical Applications

• Protein and peptide separations

• Biomolecule HPLC workflows

• Preparative method development

• Legacy USP L1 large‑molecule methods

• Long‑chain fatty acids

• Analytical to preparative scale‑up workflows

Important Usage Notes

• Designed for large molecule separations using 300 Å pore media

• Compatible with standard HPLC systems

• Ideal for maximum‑resolution biomolecule separations

• Use when method fidelity and separation performance are critical