CUSTOM ITEM: HPLC Column, RP C18, Wide Pore, 10um, 4.0mm ID x 100mm Length, 300A. Cogent Brand. 1 EA.
Additional Info:
The 68018‑10P‑4‑3M Cogent RP C18 Wide Pore HPLC Column is designed for method development and preparative scouting of large molecular weight analytes, combining 10 µm particles with a 300 Å wide‑pore structure to deliver enhanced analyte accessibility, improved recovery, and consistent chromatographic performance.
Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding and fully double end‑capped, this stationary phase provides:
Reduced silanol interactions
Improved peak shape for proteins, peptides, and large polar compounds
Reliable and reproducible chromatography
The 300 Å pore size enables biomolecules to fully access the stationary phase, minimizing restricted diffusion and improving recovery compared to standard 100 Å media.
This column is ideal as a USP L1 alternate for large molecule applications and as a scouting platform for preparative method scale‑up.
100 mm Length – Method Development & Increased Resolution
Higher resolving power than 50 mm scouting columns
Improved separation of proteins, peptides, and complex biomolecules
Increased peak capacity for method optimization
Ideal for refining separations prior to preparative scale‑up
This format is selected when greater resolution is needed beyond initial scouting runs.
4.0 mm ID – Solvent‑Saving Analytical Format
Comparable chromatographic behavior to 4.6 mm ID columns
Reduces mobile phase consumption and waste
Typical flow rate range: ~0.8–1.2 mL/min
Optimal starting range: ~0.9–1.1 mL/min
Benefits:
Lower solvent consumption
Reduced operating cost
Compatible with standard HPLC systems
Flow Rate & System Considerations (4.0 mm ID, 10 µm, 300 Å)
Suitable for standard HPLC systems
Supports higher flow rates and increased sample loading
Well suited for:
Preparative method development
Biomolecule scouting workflows
Scale‑up method optimization
Why Choose Wide Pore (300 Å) C18 (10 µm)
Designed for Large Molecules
Enables separation of:
Proteins
Peptides
Large biomolecules
Eliminates mass transfer limitations of 100 Å phases
Ideal for Preparative Scouting
Used to develop and optimize methods prior to preparative scale‑up
Predictable selectivity when transferring to larger preparative columns
Supports efficient analytical‑to‑prep workflow development
Maintains Reversed‑Phase Selectivity
Retains classic C18 hydrophobic interaction behavior
Suitable for lipids and long‑chain fatty acids
Cogent RP C18™ Advantages
Fully End‑Capped Surface
Minimizes secondary interactions
Improves peak symmetry
Better performance for basic analytes
High Purity Silica
Consistent chromatographic performance
Reduced variability between runs
Durable & Cost‑Efficient
Long column lifetime
Ideal for repeated method development cycles
Lower maintenance frequency
Typical Applications
Preparative biomolecule scouting
Protein and peptide separations
Method development and optimization
Hydrophobic and neutral compound separations
Long‑chain fatty acids
Analytical to preparative scale‑up workflows
USP L1 wide‑pore reversed‑phase methods
Important Usage Notes
Designed for large molecule separations using 300 Å pore media
Compatible with standard HPLC systems
Ideal for method refinement prior to preparative scale‑up
Choose when robustness and loading capacity are prioritized over efficiency
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable