Catalog Number - 68018-10P-3M

The 68018‑10P‑3M Cogent RP C18 Wide Pore HPLC Column is designed for method development and preparative scouting of large molecular weight analytes, combining 10 µm particles with a 300 Å wide‑pore structure to deliver enhanced analyte accessibility, reliable retention, and consistent chromatographic performance.

Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding and fully double end‑capped, this stationary phase provides:

• Reduced silanol interactions

• Improved peak shape for proteins, peptides, and large polar compounds

• Consistent and reproducible chromatography

The 300 Å pore size enables large molecules to fully access the stationary phase, minimizing restricted diffusion and improving recovery compared to standard 100 Å columns.

This column is ideal as a USP L1 alternate for large molecule applications and as a scouting platform for preparative method development.

100 mm Length – Method Development & Increased Resolution

• Higher resolving power than 50 mm scouting columns

• Improved separation of biomolecules and closely related analytes

• Increased peak capacity during method optimization

• Ideal for refining separations prior to preparative scale‑up

This configuration is selected when greater resolution is required beyond initial screening conditions.

4.6 mm ID – Standard Analytical Performance

• Industry‑standard HPLC column dimension

• Compatible with all conventional HPLC systems

• Typical flow rate range: ~0.8–1.5 mL/min

• Optimal starting range: ~1.0–1.2 mL/min

Provides:

• Strong detector response

• Reliable reproducibility

• Easy method transfer across laboratories

Flow Rate & System Considerations (4.6 mm ID, 10 µm, 300 Å)

• Suitable for standard HPLC systems

• Supports higher flow rates and increased sample loading

• Well suited for:

  • Preparative method development

  • Biomolecule scouting workflows

  • Scale‑up method optimization

Why Choose Wide Pore (300 Å) C18 (10 µm)

Designed for Large Molecules

• Enables efficient separation of:

  • Proteins

  • Peptides

  • Large biomolecules

• Eliminates mass transfer limitations of 100 Å stationary phases

Ideal for Preparative Scouting

• Used to develop and optimize methods prior to preparative scale‑up

• Predictable selectivity when transitioning to larger preparative columns

• Supports efficient analytical‑to‑preparative workflows

Maintains Reversed‑Phase Selectivity

• Retains classic C18 hydrophobic interaction behavior

• Suitable for lipids and long‑chain fatty acids

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry

• Better performance for basic analytes

High Purity Silica

• Consistent chromatographic performance

• Reduced variability between runs

Durable & Cost‑Efficient

• Long column lifetime

• Ideal for repeated method development

• Lower maintenance frequency

Typical Applications

• Preparative biomolecule scouting

• Protein and peptide separations

• Method development and optimization

• Hydrophobic and neutral compound separations

• Long‑chain fatty acids

• Analytical to preparative scale‑up workflows

• USP L1 wide‑pore reversed‑phase methods

Important Usage Notes

• Designed for large molecule separations using 300 Å pore media

• Compatible with standard HPLC systems

• Ideal for method development prior to scale‑up

• Choose when loading capacity and robustness are prioritized over efficiency