CUSTOM ITEM: HPLC Column, RP C18, Wide Pore, 10um, 4.0mm ID x 50mm Length, 300A. Cogent Brand. 1 EA.
Additional Info:
The 68018‑05P‑4‑3M Cogent RP C18 Wide Pore HPLC Column is designed for method development and preparative scouting of large molecular weight analytes, combining 10 µm particles with a 300 Å wide‑pore structure to deliver robust performance, enhanced analyte accessibility, and reproducible results.
Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding and fully double end‑capped, this stationary phase provides:
Reduced silanol interactions
Improved peak shape for proteins, peptides, and large polar compounds
Consistent and durable chromatographic performance
The 300 Å pore size enables biomolecules to fully access the stationary phase, minimizing restricted diffusion and improving recovery compared to standard 100 Å columns.
This column is widely used as a USP L1 alternate for large molecules and as a scouting platform for preparative method scale‑up.
50 mm Length – Fast Biomolecule Scouting
Designed for rapid separations and short run times
Ideal for early‑stage biomolecule method development
Minimizes solvent consumption during screening
Excellent for high‑throughput preparative scouting workflows
4.0 mm ID – Solvent‑Saving Analytical Format
Comparable chromatographic behavior to 4.6 mm ID columns
Reduces mobile phase consumption and waste
Typical flow rate range: ~0.8–1.2 mL/min
Optimal starting range: ~0.9–1.1 mL/min
Benefits:
Lower solvent cost
Reduced waste generation
Compatible with standard HPLC systems
Flow Rate & System Considerations (4.0 mm ID, 10 µm, 300 Å)
Suitable for standard HPLC systems
Supports higher flow rates and increased sample loading
Well suited for:
Preparative method development
Biomolecule scouting workflows
Scale‑up method optimization
Why Choose Wide Pore (300 Å) C18 (10 µm)
Designed for Large Molecules
Enables separation of:
Proteins
Peptides
Large biomolecules
Eliminates mass transfer limitations of 100 Å phases
Ideal for Preparative Scouting
Used to develop methods prior to preparative scale‑up
Predictable selectivity when transitioning to larger ID prep columns
Supports efficient analytical‑to‑prep workflow development
Maintains Reversed‑Phase Selectivity
Retains classic C18 hydrophobic behavior
Suitable for lipids and long‑chain fatty acids
Cogent RP C18™ Advantages
Fully End‑Capped Surface
Minimizes secondary interactions
Improves peak symmetry
Better performance for basic analytes
High Purity Silica
Consistent chromatographic performance
Reduced variability between runs
Durable & Cost‑Efficient
Long column lifetime
Ideal for repeated method development
Reduced maintenance frequency
Typical Applications
Preparative biomolecule scouting
Protein and peptide separations
Method development and optimization
Hydrophobic and neutral compound separations
Long‑chain fatty acids
Analytical to preparative scale‑up workflows
USP L1 wide‑pore reversed‑phase methods
Important Usage Notes
Designed for large molecule separations using 300 Å pore media
Compatible with standard HPLC systems
Ideal for fast screening prior to preparative scale‑up
Choose when sample loading capacity and robustness are prioritized over efficiency
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable