Catalog Number - 67590-25P

Cogent IEX WAX is a weak anion exchange (WAX) HPLC stationary phase designed for the retention and separation of negatively charged compounds. Manufactured using high-purity silica and diethylaminopropyl functional groups, the stationary phase provides a positively charged surface within its functional pH range, delivering reliable anion exchange selectivity for acidic compounds and other anionic analytes. This popular chromatography phase has been widely used in HPLC laboratories for many years and serves as an excellent alternative for many USP L9 methods.

67590-25P is a 250 mm x 4.6 mm ID analytical HPLC column packed with 5 µm WAX particles with a 100 Å pore size. The extended 250 mm length provides maximum resolving power, increased theoretical plate counts, and enhanced separation of closely related compounds in demanding ion exchange applications. The 4.6 mm ID format is the industry standard for conventional HPLC systems and typically operates at approximately 1.0–2.0 mL/min, making it suitable for routine analytical methods, impurity profiling, and complex separations requiring superior chromatographic resolution.

This weak anion exchange column is particularly useful for the analysis and purification of organic acids, pharmaceuticals, biomolecules, and other negatively charged compounds. The combination of selective WAX functionality, high-purity silica, efficient 5 µm particles, and extended column length provides reproducible chromatography and exceptional performance for challenging anion exchange separations where maximum resolution is required.

Key Benefits

• Weak anion exchange (WAX) stationary phase

• Diethylaminopropyl functional groups

• Suitable for negatively charged analytes

• Alternative for many USP L9 methods

• High-purity silica support

• 5 µm particles for efficient separations

• Maximum chromatographic resolution from 250 mm column length

• Increased theoretical plate counts

• Excellent reproducibility and ion exchange selectivity

Typical Applications

• Ion exchange chromatography

• Anion separations

• Acidic compound analysis

• Organic acid analysis

• Pharmaceutical analysis

• USP L9 methods

• Impurity profiling

• Method validation

• Purification of negatively charged compounds

• Complex sample separations

Specifications

• Product Type: HPLC Column

• Stationary Phase: IEX WAX

• USP Classification: L9

• Chromatography Mode: Weak Anion Exchange

• Functional Group: Diethylaminopropyl

• Particle Size: 5 µm

• Pore Size: 100 Å

• Column Dimensions: 4.6 mm ID x 250 mm

• Length: 250 mm

• Recommended Flow Rate: 1.0–2.0 mL/min

• Brand: Cogent

Important Notes

• Designed for weak anion exchange chromatography

• Retains negatively charged analytes

• Functional charged range approximately pH 5–8

• Suitable for many USP L9 applications

• High-purity silica provides reliable chromatographic performance

• Longer column length provides maximum chromatographic resolution

• Ideal for impurity profiling and complex separations

• Compatible with conventional HPLC systems