Catalog Number - 64424-25P

Nova Similars Phenyl™ is a traditional reversed-phase HPLC stationary phase manufactured on high-purity Type-B spherical silica with a fully endcapped phenyl bonded phase. The stationary phase consists of a four-carbon linker terminating in a phenyl functional group, providing unique chromatographic selectivity through a combination of hydrophobic and π-π interactions. Designed as a cost-effective alternative to Waters™ Nova-Pak® Phenyl columns, it offers comparable chromatographic behavior for USP L11 methods while delivering the reproducibility and reliability required for routine analytical, quality control, and method-transfer applications.

64424-25P is a 250 mm x 4.6 mm ID analytical column packed with 4 µm Nova Similars Phenyl particles. The extended 250 mm length provides increased theoretical plate counts and enhanced resolving power for impurity profiling, complex sample matrices, and challenging separations where maximum resolution is required. The 4 µm particle size provides improved efficiency and sharper peak shapes than comparable 5 µm columns while maintaining moderate operating pressure on conventional HPLC systems. Typical operating flow rates are approximately 1.0–2.0 mL/min.

This USP L11 column is particularly useful for aromatic compounds where phenyl selectivity can provide different retention and separation characteristics than traditional C18 phases. Common applications include pharmaceutical analysis, aromatic hydrocarbons, environmental testing, impurity profiling, and methods requiring enhanced aromatic selectivity through π-π interactions.

Key Benefits

• Type-B spherical silica stationary phase

• Suitable alternative for USP L11 methods

• Comparable selectivity to Waters™ Nova-Pak® Phenyl columns

• Fully endcapped phenyl bonded phase

• Enhanced aromatic compound selectivity

• Unique π-π interaction mechanism

• Higher efficiency than comparable 5 µm columns

• Increased resolution from 250 mm column length

Typical Applications

• USP L11 methods

• Reversed-phase HPLC

• Pharmaceutical analysis

• Aromatic compound separations

• Environmental testing

• Impurity profiling

• Method development

• Method transfer and validation

Specifications

• Product Type: HPLC Column

• Stationary Phase: Nova Similars Phenyl

• USP Classification: L11

• Particle Size: 4 µm

• Particle Type: Type-B Spherical Silica

• Bonded Phase: Phenyl

• Endcapped: Fully

• Column Dimensions: 4.6 mm ID x 250 mm

• Length: 250 mm

• Recommended Flow Rate: 1.0–2.0 mL/min

• Brand: Cogent

Important Notes

• Designed for reversed-phase chromatography

• Phenyl selectivity provides different retention than C18 phases

• Enhanced interactions with aromatic compounds

• Longer column length provides increased resolving power

• Higher efficiency than comparable 5 µm columns

• Moderate operating pressure on conventional HPLC systems

• Excellent alternative for many USP L11 methods

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