Catalog Number - 62518-10P-2

Hyper Similars C18™ is a traditional reversed-phase HPLC stationary phase manufactured on high-purity Type-B spherical silica with a fully endcapped octadecyl (C18) bonded phase. Designed as a cost-effective alternative to legacy Hypersil® and Spherisorb® columns, it offers comparable chromatographic behavior for USP L1 methods while providing the strong hydrophobic retention, reproducibility, and broad applicability expected from a conventional C18 stationary phase.

62518-10P-2 is a 100 mm x 2.1 mm ID narrow-bore analytical column packed with 5 µm, 150Å Hyper Similars C18 particles. The 100 mm length provides a balance of analysis speed and chromatographic resolution, while the 2.1 mm ID format reduces solvent consumption and is ideally suited for LC-MS applications. Typical operating flow rates are approximately 0.2–0.3 mL/min and require a system capable of precise low-flow delivery with low-volume flow cells and plumbing. The 5 µm particle size provides reliable performance with moderate operating pressure.

This USP L1 column is ideal for hydrophobic and neutral compounds requiring strong reversed-phase retention. Its narrow-bore design makes it a popular choice for LC-MS methods, pharmaceutical analysis, long-chain fatty acid separations, and routine analytical workflows where sensitivity, efficiency, and solvent savings are important.

Key Benefits

• Type-B spherical silica stationary phase

• Suitable alternative for USP L1 methods

• Comparable selectivity to Hypersil® and Spherisorb® C18 columns

• Narrow-bore format ideal for LC-MS applications

• Balanced speed and resolution with 100 mm length

• Reliable performance with 5 µm particles

• Reduced solvent consumption

• End-capped C18 bonded phase

Typical Applications

• USP L1 methods

• LC-MS analysis

• Reversed-phase HPLC

• Pharmaceutical analysis

• Long-chain fatty acid analysis

• Quality control testing

• Method development

• Method transfer and validation

Specifications

• Product Type: HPLC Column

• Stationary Phase: Hyper Similars C18

• USP Classification: L1

• Particle Size: 5 µm

• Particle Type: Type-B Spherical Silica

• Bonded Phase: C18 (Octadecyl)

• Endcapped: Yes

• Pore Size: 150Å

• Column Dimensions: 2.1 mm ID x 100 mm

• Length: 100 mm

• Recommended Flow Rate: 0.2–0.3 mL/min

• Brand: Cogent

Important Notes

• Designed for reversed-phase chromatography

• Narrow-bore format optimized for LC-MS applications

• Requires an HPLC or LC-MS system capable of reliable low-flow operation

• Lower operating pressure than comparable 3 µm columns

• Reduced solvent consumption compared to 4.6 mm ID columns

• Excellent alternative for many USP L1 methods

• Ideal for high-sensitivity analyses

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