Catalog Number - 59305-03-4

MicroBee C18™ is a traditional octadecylsilane (C18) reversed-phase HPLC stationary phase that has been trusted by chromatographers for many years. As part of the Cogent™ Similars product line, it provides a cost-effective alternative for established analytical methods while delivering the hydrophobic retention characteristics expected from classical C18 chemistry. The end-capped, Type-A silica support is bonded with a straight-chain C18 ligand, making it particularly useful for laboratories operating legacy methods and compendial procedures.

59305-03-4 is a 300 mm x 4.6 mm ID analytical column packed with 5 µm, 125Å MicroBee C18 particles. The extended 300 mm column length is designed for applications where maximum chromatographic resolution is required, providing increased theoretical plates and improved separation of closely related compounds. The 5 µm particle size delivers greater efficiency and sharper peak shapes than 10 µm packings, while the industry-standard 4.6 mm ID format typically operates optimally at approximately 1.0–2.0 mL/min on conventional HPLC systems.

This USP L1 column is an excellent alternative for methods developed on Microbondapak™ and similar C18 columns. The traditional C18 chemistry is well suited for hydrophobic and neutral compounds analyzed under reversed-phase conditions and is particularly effective for long-chain fatty acids, pharmaceutical compounds, and other lipophilic analytes. When maximum separation is more important than analysis speed, the combination of a 300 mm length and 5 µm particle size provides the additional resolving power often required for complex samples and impurity profiling.

Key Benefits

• Traditional end-capped C18 stationary phase

• Suitable alternative for USP L1 methods

• Compatible with many legacy Microbondapak™ methods

• Higher efficiency than comparable 10 µm columns

• Maximum resolution from 300 mm column length

• Improved peak shape and separation performance

• Excellent for hydrophobic and neutral compounds

• Cost-effective Cogent Similars column

Typical Applications

• USP L1 methods

• Reversed-phase HPLC

• Complex sample separations

• Pharmaceutical analysis

• Impurity profiling

• Long-chain fatty acid analysis

• Lipophilic compound separations

• Method development and optimization

Specifications

• Product Type: HPLC Column

• Stationary Phase: MicroBee C18

• USP Classification: L1

• Particle Size: 5 µm

• Particle Type: Type-A Silica

• Bonded Phase: C18 (Octadecylsilane)

• Endcapped: Yes

• Pore Size: 125Å

• Column Dimensions: 4.6 mm ID x 300 mm

• Length: 300 mm

• Recommended Flow Rate: 1.0–2.0 mL/min

• Brand: Cogent

Important Notes

• Designed for reversed-phase chromatography

• Traditional end-capped C18 chemistry

• 5 µm particles provide higher efficiency and sharper peaks

• Compatible with conventional HPLC systems

• Industry-standard 4.6 mm ID analytical format

• Extended column length provides maximum resolving power

• Ideal when additional separation is required

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