Catalog Number - 59300-05

MicroBee Silica™ is an unbonded, Type-A silica HPLC stationary phase designed for normal phase separations using traditional adsorption chromatography mechanisms. As part of the Cogent™ Similars product line, it has been a trusted value-oriented choice for laboratories running established silica methods for many years. The untreated silica surface provides strong interactions with polar analytes while offering broad applicability for normal phase chromatography and legacy method replacement.

59300-05 is a 300 mm x 3.9 mm ID analytical column packed with 10 µm, 125Å MicroBee Silica particles. This dimension closely matches the format used in many historical HPLC methods developed on Porasil® and similar silica columns, making it particularly useful for method transfer and replacement applications. The 300 mm length provides maximum resolving power and increased theoretical plate counts for difficult separations, while the 3.9 mm ID format typically performs well at flow rates of approximately 0.8–1.5 mL/min on conventional HPLC systems.

Because the stationary phase consists of completely unmodified silica, this USP L3 column is well suited for legacy normal phase methods involving hydrophilic, hydrophobic, polar, and non-polar compounds. It offers selectivity comparable to traditional Porasil® silica columns while providing the flexibility expected from an unbonded stationary phase. Unmodified silica is also suitable for HILIC applications when operated with appropriate high-organic mobile phase conditions.

Key Benefits

• Unmodified Type-A silica stationary phase

• Suitable alternative for USP L3 method requirements

• Compatible with many legacy silica methods

• Maximum resolution from 300 mm column length

• Ideal for normal phase chromatography

• Suitable for HILIC applications

• 125Å pore size for broad analyte compatibility

• Cost-effective Cogent Similars column

Typical Applications

• USP L3 methods

• Normal phase HPLC

• HILIC separations

• Pharmaceutical analysis

• Method development

• Impurity profiling

• Quality control testing

• Polar compound separations

Specifications

• Product Type: HPLC Column

• Stationary Phase: MicroBee Silica

• USP Classification: L3

• Particle Size: 10 µm

• Particle Type: Type-A Silica

• Pore Size: 125Å

• Column Dimensions: 3.9 mm ID x 300 mm

• Length: 300 mm

• Recommended Flow Rate: 0.8–1.5 mL/min

• Brand: Cogent

Important Notes

• Designed for normal phase chromatography

• Suitable for HILIC methods with appropriate mobile phases

• Unmodified silica stationary phase

• Ideal for legacy method replacement

• Compatible with conventional HPLC systems

• Provides selectivity comparable to traditional Porasil® silica columns

• Extended column length provides maximum resolving power

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