Catalog Number - 59300-02

MicroBee Phenyl™ is a traditional phenyl-bonded HPLC stationary phase designed to provide selectivity distinct from conventional C18 columns. As part of the Cogent™ Similars product line, it has been a trusted value-oriented option for laboratories using established phenyl methods for many years. The fully endcapped phenyl bonded phase is attached to Type-A silica and is particularly useful for aromatic compounds where π-π interactions can provide selectivity advantages over standard alkyl phases.

59300-02 is a 300 mm x 3.9 mm ID analytical column packed with 10 µm, 125Å MicroBee Phenyl particles. This dimension closely matches the format used in many historical HPLC methods developed on Microbondapak™ and similar phenyl columns, making it especially valuable for method transfer and replacement applications. The 300 mm length provides maximum resolving power and increased theoretical plate counts for challenging separations where chromatographic resolution is more important than analysis speed. The 3.9 mm ID format typically performs well at flow rates of approximately 0.8–1.5 mL/min on conventional HPLC systems.

This USP L11 column is an excellent alternative for methods developed on Microbondapak™ Phenyl and similar phenyl stationary phases. The phenyl functionality offers enhanced retention and selectivity for aromatic, conjugated, and structurally related compounds while maintaining the versatility needed for a wide range of reversed-phase separations. When C18 columns do not provide adequate separation of closely related analytes, the combination of phenyl selectivity and extended column length can provide the additional resolution needed.

Key Benefits

• Fully endcapped phenyl stationary phase

• Suitable alternative for USP L11 methods

• Compatible with many legacy Microbondapak™ methods

• Enhanced selectivity for aromatic compounds

• Maximum resolution from 300 mm column length

• Different retention characteristics than C18 columns

• 125Å pore size for broad analyte compatibility

• Cost-effective Cogent Similars column

Typical Applications

• USP L11 methods

• Reversed-phase HPLC

• Aromatic compound analysis

• Pharmaceutical analysis

• Impurity profiling

• Method development

• Isomer separations

• Complex sample separations

Specifications

• Product Type: HPLC Column

• Stationary Phase: MicroBee Phenyl

• USP Classification: L11

• Particle Size: 10 µm

• Particle Type: Type-A Silica

• Bonded Phase: Phenyl

• Endcapped: Yes

• Pore Size: 125Å

• Column Dimensions: 3.9 mm ID x 300 mm

• Length: 300 mm

• Recommended Flow Rate: 0.8–1.5 mL/min

• Brand: Cogent

Important Notes

• USP L11 column classification

• Fully endcapped phenyl stationary phase

• Designed for reversed-phase chromatography

• Ideal for legacy method replacement

• Compatible with conventional HPLC systems

• Extended column length provides maximum resolving power

• Excellent alternative for established phenyl methods

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