Catalog Number - 59052-25P

MicroBee Phenyl™ is a traditional phenyl-bonded HPLC stationary phase designed to provide selectivity that differs from conventional C18 columns. As part of the Cogent™ Similars product line, it has been a trusted value-oriented option for laboratories using established phenyl methods for many years. The fully endcapped phenyl bonded phase is attached to Type-A silica and is particularly useful for aromatic compounds where π-π interactions can provide selectivity advantages over standard alkyl phases.

59052-25P is a 250 mm x 4.6 mm ID analytical column packed with 10 µm, 125Å MicroBee Phenyl particles. The 250 mm length delivers increased resolving power and higher theoretical plate counts than shorter columns, making it well suited for complex separations, impurity profiling, and methods requiring maximum chromatographic resolution. The industry-standard 4.6 mm ID format typically operates optimally at flow rates of approximately 1.0–2.0 mL/min on conventional HPLC systems.

This USP L11 column is an excellent alternative for methods developed on Microbondapak™ Phenyl and similar phenyl stationary phases. The phenyl functionality offers enhanced retention and selectivity for aromatic, conjugated, and structurally related compounds while maintaining the versatility needed for a wide range of reversed-phase separations. It is often selected during method development when C18 columns do not provide adequate resolution between closely related analytes.

Key Benefits

• Fully endcapped phenyl stationary phase

• Suitable alternative for USP L11 methods

• Enhanced selectivity for aromatic compounds

• Increased resolution from 250 mm column length

• Different retention characteristics than C18 columns

• 125Å pore size for broad analyte compatibility

• Cost-effective Cogent Similars column

• Compatible with legacy phenyl methods

Typical Applications

• USP L11 methods

• Reversed-phase HPLC

• Aromatic compound analysis

• Pharmaceutical analysis

• Impurity profiling

• Method development

• Isomer separations

• Alternative selectivity screening

Specifications

• Product Type: HPLC Column

• Stationary Phase: MicroBee Phenyl

• USP Classification: L11

• Particle Size: 10 µm

• Particle Type: Type-A Silica

• Bonded Phase: Phenyl

• Endcapped: Yes

• Pore Size: 125Å

• Column Dimensions: 4.6 mm ID x 250 mm

• Length: 250 mm

• Recommended Flow Rate: 1.0–2.0 mL/min

• Brand: Cogent

Important Notes

• USP L11 column classification

• Fully endcapped phenyl stationary phase

• Compatible with conventional HPLC systems

• Industry-standard 4.6 mm ID analytical format

• Longer column length provides greater resolving power

• Suitable for aromatic and conjugated compounds

• Excellent replacement for many legacy phenyl methods

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