Catalog Number - 59052-05-4

MicroBee Silica™ is an unbonded, Type-A silica HPLC stationary phase designed for normal phase separations using traditional adsorption chromatography mechanisms. As part of the Cogent™ Similars product line, it has been a trusted value-oriented choice for laboratories running established silica methods for many years. The untreated silica surface provides strong interactions with polar analytes while offering broad applicability for normal phase chromatography and method transfer from legacy silica columns.

59052-05-4 is a 300 mm x 4.6 mm ID analytical column packed with 10 µm, 125Å MicroBee Silica particles. The extended 300 mm column length maximizes resolving power and theoretical plate count, making it an excellent choice for difficult separations, impurity profiling, and applications where maximum chromatographic resolution is more important than analysis speed. The industry-standard 4.6 mm ID format typically operates optimally at approximately 1.0–2.0 mL/min on conventional HPLC systems.

Because the stationary phase consists of completely unmodified silica, this column is particularly well suited as an alternative for legacy methods specifying USP L3 columns. It can be used for hydrophilic, hydrophobic, polar, and non-polar compounds under normal phase conditions and offers performance comparable to traditional Porasil® silica columns. Unbonded silica is also suitable for HILIC applications when operated with appropriate high-organic mobile phase conditions.

Key Benefits

• Unmodified Type-A silica stationary phase

• Suitable alternative for USP L3 method requirements

• Maximum resolution from 300 mm column length

• Ideal for normal phase chromatography

• Suitable for HILIC applications

• 125Å pore size for broad analyte compatibility

• Cost-effective Cogent Similars column

• Comparable to traditional silica columns

Typical Applications

• USP L3 methods

• Normal phase HPLC

• HILIC separations

• Pharmaceutical analysis

• Method development

• Impurity profiling

• Quality control testing

• Polar compound separations

Specifications

• Product Type: HPLC Column

• Stationary Phase: MicroBee Silica

• USP Classification: L3

• Particle Size: 10 µm

• Particle Type: Type-A Silica

• Pore Size: 125Å

• Column Dimensions: 4.6 mm ID x 300 mm

• Length: 300 mm

• Recommended Flow Rate: 1.0–2.0 mL/min

• Brand: Cogent

Important Notes

• Designed for normal phase chromatography

• Suitable for HILIC methods with appropriate mobile phases

• Unmodified silica stationary phase

• Industry-standard 4.6 mm ID analytical format

• Compatible with conventional HPLC systems

• Extended column length provides maximum resolving power

• Alternative option for many legacy USP L3 methods

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