Catalog Number - 59001-15P

MicroBee Silica™ is an unbonded, Type-A silica HPLC stationary phase designed for normal phase separations using traditional silica chromatography mechanisms. As part of the Cogent™ Similars product line, these columns provide a cost-effective alternative for laboratories running established silica methods while maintaining reliable chromatographic performance. The untreated silica surface offers strong interactions with polar analytes, making it suitable for a broad range of normal phase applications.

59001-15P is a 150 mm x 4.6 mm ID analytical column packed with 10 µm, 125Å MicroBee Silica particles. The 150 mm length provides a balance of efficiency and analysis time, making it well suited for routine method development, quality control, and compendial testing. The 4.6 mm ID format is the industry-standard analytical dimension and typically operates optimally at flow rates of approximately 1.0–2.0 mL/min on conventional HPLC systems.

Because the stationary phase consists of unmodified silica, this column is particularly useful as an alternative for legacy methods specifying USP L3 silica columns. It is suitable for separating hydrophilic, hydrophobic, polar, and non-polar compounds under normal phase conditions and offers performance comparable to traditional Porasil® silica columns. Unbonded silica is also suitable for HILIC applications when appropriate mobile phase conditions are employed.

Key Benefits

• Unmodified Type-A silica stationary phase

• Suitable alternative for USP L3 method requirements

• Ideal for normal phase chromatography

• Suitable for HILIC applications

• 125Å pore size for a wide range of analytes

• Cost-effective Cogent Similars column

• Comparable performance to traditional silica columns

• Compatible with established and legacy methods

Typical Applications

• USP L3 methods

• Normal phase HPLC

• HILIC separations

• Pharmaceutical analysis

• Raw material testing

• Quality control laboratories

• Method transfer and validation

• Polar compound separations

Specifications

• Product Type: HPLC Column

• Stationary Phase: MicroBee Silica

• USP Classification: L3

• Particle Size: 10 µm

• Particle Type: Type-A Silica

• Pore Size: 125Å

• Column Dimensions: 4.6 mm ID x 150 mm

• Length: 150 mm

• Recommended Flow Rate: 1.0–2.0 mL/min

• Brand: Cogent

Important Notes

• Designed for normal phase chromatography

• Suitable for HILIC methods with appropriate mobile phases

• Unmodified silica stationary phase

• Industry-standard 4.6 mm ID analytical format

• Compatible with conventional HPLC systems

• Alternative option for many legacy USP L3 methods

• Comparable to traditional Porasil® silica columns

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