Caffeine Test Mixture Solution. 30 ml. Chemical Solutions brand. 1 EA.
Additional Info:
These mixtures are designed for use in HPLC system suitability testing, column performance verification, and method development.
Key Features
Primary Component: Caffeine, a well-characterized compound with strong UV absorbance, making it ideal for UV detection in HPLC.
Application: Used to evaluate column efficiency, resolution, and reproducibility in reversed-phase HPLC systems.
Detection: Typically monitored at 205 nm or 273 nm maximum, due to caffeine’s strong UV absorbance at this wavelength.
Formulation: Supplied in a ready-to-use solution, often in a mixture of water and organic solvent (e.g., acetonitrile or methanol), though exact composition may vary by lot.
Common Uses
System Suitability Testing: Ensures that the HPLC system is performing within acceptable parameters.
Column Testing: Assesses column performance, including retention time, peak shape, and resolution.
Training and Method Development: Provides a reliable standard for training analysts or developing new methods.
Related Technical Notes
Spectrum of Caffeine in Resolution Test Mixture (RTM) provides UV absorbance data and chromatographic behavior of caffeine in the RTM.
Application Note - discusses caffeine quantification in beverages using HPLC, which may use similar standards for calibration.
*This item can be made to order and may have a lead-time of 10-15 business days.