Catalog Number - 42100-10P-2

The 42100‑10P‑2 Method Development Kit 2102 is an application‑intensive, multi‑phase column kit designed to support comprehensive method development across HILIC and reversed‑phase chemistries using Cogent TYPE‑C™ silica hydride technology. This kit provides a cost‑effective way to evaluate five complementary stationary phases commonly used in modern LC‑MS workflows, all in a matched 2.1 mm × 100 mm, 2.o format.

Each column in the kit is manufactured with Cogent TYPE‑C™ silica hydride, delivering fast equilibration, stable retention behavior, and robust lifetime performance under demanding LC‑MS conditions.

Kit Composition

This method‑development kit includes five analytical HPLC columns, each offering distinct selectivity while sharing identical physical specifications for direct comparison:

• Cogent Diamond Hydride™ 2.o

• Cogent Bidentate C18™ 2.o

• Cogent Bidentate C8™ 2.o

• Cogent Silica‑C™ 2.o

• Cogent UDC‑Cholesterol™ 2.o

Shared specifications for all columns:

• Internal Diameter: 2.1 mm

• Length: 100 mm

• Particle Size: 2.2 µm

• Pore Size: 120 Å

• Stationary Phase Platform: Cogent TYPE‑C™ silica hydride

Using a fully matched geometry and particle platform eliminates variability due to column hardware, allowing selectivity differences to be attributed solely to stationary‑phase chemistry.

Application Focus: Broad‑Spectrum Method Development for HILIC & RP

This kit is designed for systematic method development, screening, and optimization across a wide range of analyte polarities:

Column Selectivity Coverage

• Diamond Hydride™ 2.o – HILIC‑style retention for highly polar analytes

• Silica‑C™ 2.o – Normal‑phase / HILIC behavior for non‑aqueous separations

• Bidentate C18™ 2.o – Reversed‑phase retention for moderately polar to hydrophobic compounds

• Bidentate C8™ 2.o – Reduced hydrophobicity vs C18 for altered RP selectivity

• UDC‑Cholesterol™ 2.o – Shape‑selective RP phase for aromatic and sterically distinct analytes

Together, these columns enable rapid selectivity scouting across HILIC and reversed‑phase modes using a single particle architecture and surface chemistry.

Why Choose the 2.1 mm × 100 mm 2.o Format

The 2.1 mm ID × 100 mm length combined with 2.2 µm particles provides an excellent balance of resolution, speed, and LC‑MS compatibility:

• Higher peak capacity than 50 mm formats

• Near‑UHPLC efficiency with manageable backpressure

• Reduced solvent consumption compared to 4.6 mm columns

• Ideal for LC‑MS and LC‑MS/MS workflows requiring consistent low‑flow operation

This format is widely used for analytical screening prior to scale‑up or phase selection.

Key Advantages of Cogent TYPE‑C™ Technology

Across all five columns, the TYPE‑C™ silica hydride platform provides:

• Faster equilibration than conventional bonded phases

• Improved retention stability under high‑organic and aqueous conditions

• Robust column lifetime across diverse mobile‑phase systems

• Consistent behavior across HILIC and RP modes, reducing retraining effort

These attributes simplify comparison and reduce method‑development time.

System Requirements and Flow Rates

• Typical flow range (2.1 mm ID): ~0.2–0.3 mL/min

• Requires LC or UHPLC systems capable of stable, accurate low‑flow delivery

• Optimized for LC‑MS and LC‑MS/MS platforms

Final operating conditions should be optimized based on mobile‑phase composition, temperature, and system pressure limits.