Method Development Kit 2154, TYPE-C. Includes 5 Cogent HPLC Columns, 2.1mm x 150mm. Cogent TYPE-C Brand. 1 PK.
Additional Info:
The 40100‑15P‑2 Method Development Kit 2154 is a high‑resolution, multi‑column method‑development and selectivity‑scouting kit built on Cogent TYPE‑C™ silica hydride technology. This kit provides an economical way to evaluate five complementary stationary phases commonly used in HILIC and reversed‑phase LC‑MS workflows, all in a matched 2.1 mm × 150 mm analytical format.
Each column in the kit is manufactured with Cogent TYPE‑C™ silica hydride, delivering fast equilibration, stable retention behavior, and robust lifetime performance across a wide range of mobile‑phase conditions frequently used in LC‑MS.
Kit Composition
This method‑development kit includes five analytical HPLC columns, each offering distinct selectivity while sharing identical physical specifications to enable direct, unbiased comparison:
Shared specifications for all columns:
Internal Diameter: 2.1 mm
Length: 150 mm
Particle Size: 4 µm
Pore Size: 100 Å
Stationary Phase Platform: Cogent TYPE‑C™ silica hydride
The fully matched geometry ensures that differences observed during method scouting reflect stationary‑phase chemistry, not column dimensions, pressure, or dwell‑volume effects.
Application Focus: Method Development & Selectivity Scouting for LC‑MS
Kit 2154 is optimized for systematic method development and selectivity evaluation in both HILIC and reversed‑phase modes, particularly for LC‑MS and LC‑MS/MS workflows requiring increased separation confidence.
Column Selectivity Coverage
Diamond Hydride™ – HILIC‑style retention for highly polar analytes
Silica‑C™ – Normal‑phase / HILIC behavior for low‑water or non‑aqueous systems
Bidentate C18™ – Reversed‑phase retention for moderately polar to hydrophobic compounds
Bidentate C8™ – Reduced hydrophobicity vs C18 for alternative RP selectivity
UDC‑Cholesterol™ – Shape‑selective RP phase for aromatic and sterically distinct analytes
Together, these phases provide broad selectivity coverage using a consistent TYPE‑C™ surface chemistry, accelerating method optimization and comparison.
Why Choose the 2.1 mm × 150 mm Format
The 2.1 mm ID × 150 mm length configuration is selected when higher resolving power and peak capacity are required beyond 50 mm and 100 mm scouting formats:
Increased separation of closely eluting compounds
Higher peak capacity for complex mixtures
Reduced solvent consumption compared to 4.6 mm ID columns
Excellent compatibility with LC‑MS/LC‑MS/MS systems
This format is ideal for refining and confirming methods after preliminary phase selection.
Key Advantages of Cogent TYPE‑C™ Technology
Across all five columns, the TYPE‑C™ silica hydride platform delivers:
Faster equilibration than conventional bonded silica phases
Improved retention stability under both high‑organic and aqueous conditions
Robust column lifetime across HILIC and RP modes
Consistent behavior across different stationary chemistries, reducing development variability
These attributes significantly shorten development cycles and improve reproducibility.
System Requirements and Flow Rates
Typical flow range (2.1 mm ID): ~0.2–0.3 mL/min
Requires an LC or LC‑MS system capable of accurate, stable low‑flow delivery
Operating conditions should be optimized based on mobile‑phase composition, temperature, and system pressure limits.