Method Development Kit 4154, TYPE-C. Includes 5 Cogent HPLC Columns, 4.6mm x 150mm. Cogent TYPE-C Brand. 1 PK.
Additional Info:
The 40100‑15P Method Development Kit 4154 is a high‑resolution analytical method‑development and selectivity‑scouting kit built on Cogent TYPE‑C™ silica hydride technology. This kit offers an economical way to evaluate five complementary stationary phases commonly used in HILIC, reversed‑phase, and mixed‑mode LC methods, using a matched 4.6 mm × 150 mm analytical format.
All columns in the kit are manufactured with Cogent TYPE‑C™ silica hydride, providing fast equilibration, stable retention behavior, and robust lifetime performance across a wide range of mobile‑phase compositions.
Kit Composition
This method‑development kit includes five analytical HPLC columns, each providing distinct selectivity while sharing identical physical specifications for direct, unbiased comparison:
Shared specifications for all columns:
Internal Diameter: 4.6 mm
Length: 150 mm
Particle Size: 4 µm
Pore Size: 100 Å
Stationary Phase Platform: Cogent TYPE‑C™ silica hydride
The fully matched configuration ensures that differences observed during method scouting reflect true stationary‑phase selectivity, rather than artifacts from column geometry or pressure effects.
Application Focus: Analytical Method Development & Selectivity Scouting
Kit 4154 is optimized for systematic method development, selectivity evaluation, and refinement across both HILIC and reversed‑phase modes, particularly on standard analytical LC systems.
Column Selectivity Coverage
Diamond Hydride™ – HILIC‑style retention for highly polar analytes
Silica‑C™ – Normal‑phase / HILIC behavior for low‑water or non‑aqueous systems
Bidentate C18™ – Reversed‑phase retention for moderately polar to hydrophobic compounds
Bidentate C8™ – Reduced hydrophobicity for alternative RP selectivity
UDC‑Cholesterol™ – Shape‑selective RP phase for aromatic and sterically distinct analytes
Together, these columns provide comprehensive selectivity coverage using a consistent TYPE‑C™ surface chemistry.
Why Choose the 4.6 mm × 150 mm Format
The 4.6 mm ID × 150 mm length configuration is selected when higher resolving power and peak capacity are required beyond short and mid‑length scouting formats:
Improved separation of closely eluting compounds
Higher peak capacity for complex analytical samples
Standard analytical flow rates compatible with most LC systems
Well suited for UV, ELSD, CAD, and LC‑MS workflows
This format is ideal for final method refinement after stationary‑phase selection has been completed.
Key Advantages of Cogent TYPE‑C™ Technology
Across all five columns, the TYPE‑C™ silica hydride platform delivers:
Faster equilibration than conventional bonded silica phases
Improved retention stability under both aqueous and high‑organic conditions
Robust column lifetime across HILIC and RP modes
Consistent behavior across different stationary chemistries, reducing development variability
These attributes significantly speed method development and improve reproducibility.
System Requirements and Flow Rates
Typical flow range (4.6 mm ID): ~1.0–1.5 mL/min
Compatible with standard analytical HPLC and LC systems
No specialized low‑flow or UHPLC‑only equipment required
Final operating conditions should be optimized based on mobile‑phase composition, temperature, and system pressure limits.