Method Development Kit 2104, TYPE-C. Includes 5 Cogent HPLC Columns, 2.1mm x 100mm. Cogent TYPE-C Brand. 1 PK.
Additional Info:
The 40100‑10P‑2 Method Development Kit 2104 is a high‑resolution, multi‑column method‑development and selectivity‑scouting kit built on Cogent TYPE‑C™ silica hydride technology. This kit provides an economical way to evaluate five complementary stationary phases commonly used in HILIC and reversed‑phase LC‑MS workflows, all in a matched 2.1 mm × 100 mm analytical format.
Each column in the kit is manufactured with Cogent TYPE‑C™ silica hydride, delivering rapid equilibration, stable retention behavior, and robust lifetime performance across a wide range of mobile‑phase conditions frequently used in LC‑MS.
Kit Composition
This method‑development kit includes five analytical HPLC columns, each offering distinct selectivity while sharing identical physical specifications to enable direct and unbiased comparison:
Shared specifications for all columns:
Internal Diameter: 2.1 mm
Length: 100 mm
Particle Size: 4 µm
Pore Size: 100 Å
Stationary Phase Platform: Cogent TYPE‑C™ silica hydride
The fully matched geometry ensures that differences observed during method scouting reflect stationary‑phase chemistry, not column dimensions, pressure, or dwell‑volume effects.
Application Focus: Method Development & Selectivity Scouting for LC‑MS
Kit 2104 is optimized for systematic method development and selectivity evaluation in both HILIC and reversed‑phase modes, particularly for LC‑MS workflows.
Column Selectivity Coverage
Diamond Hydride™ – HILIC‑style retention for highly polar analytes
Silica‑C™ – Normal‑phase / HILIC behavior for low‑water or non‑aqueous systems
Bidentate C18™ – Reversed‑phase retention for moderately polar to hydrophobic compounds
Bidentate C8™ – Reduced hydrophobicity vs C18 for alternative RP selectivity
UDC‑Cholesterol™ – Shape‑selective RP phase for aromatic and sterically distinct analytes
Together, these phases provide broad selectivity coverage using a consistent TYPE‑C™ surface chemistry, simplifying direct comparison and accelerating method optimization.
Why Choose the 2.1 mm × 100 mm Format
The 2.1 mm ID × 100 mm length configuration is a preferred analytical format when increased resolution is required beyond short scouting columns:
Higher peak capacity than 50 mm formats
Improved separation confidence for closely eluting compounds
Reduced solvent consumption compared to 4.6 mm ID columns
Excellent compatibility with LC‑MS and LC‑MS/MS systems
This format is ideal for refining and confirming analytical methods after initial selectivity screening.
Key Advantages of Cogent TYPE‑C™ Technology
Across all five columns, the TYPE‑C™ silica hydride platform delivers:
Faster equilibration than conventional bonded silica phases
Improved retention stability under both high‑organic and aqueous conditions
Robust column lifetime across HILIC and RP modes
Consistent behavior across different stationary chemistries, reducing method‑development variability
These attributes significantly reduce development time and improve reproducibility.
System Requirements and Flow Rates
Typical flow range (2.1 mm ID): ~0.2–0.3 mL/min
Requires an LC or LC‑MS system capable of accurate, stable low‑flow delivery
Final operating conditions should be optimized based on mobile‑phase composition, temperature, and system pressure limits.