Method Development Kit 4104, TYPE-C. Includes 5 Cogent HPLC Columns, 4.6mm x 100mm. Cogent TYPE-C Brand. 1 PK.
Additional Info:
The 40100‑10P Method Development Kit 4104 is an analytical, higher‑resolution method‑development and selectivity‑scouting kit based on Cogent TYPE‑C™ silica hydride technology. This kit offers an economical way to evaluate five complementary stationary phases widely used in HILIC and reversed‑phase LC workflows, using a matched 4.6 mm × 100 mm analytical format.
All columns share identical geometry and particle characteristics, allowing chromatographers to directly compare stationary‑phase selectivity without confounding effects from column dimensions, backpressure, or dwell‑volume differences.
Kit Composition
This method‑development kit includes five analytical HPLC columns, each providing distinct selectivity while maintaining uniform physical specifications:
Shared specifications for all columns:
Internal Diameter: 4.6 mm
Length: 100 mm
Particle Size: 4 µm
Pore Size: 100 Å
Stationary Phase Platform: Cogent TYPE‑C™ silica hydride
The fully matched configuration ensures rapid, unbiased selectivity screening and method refinement.
Application Focus: Analytical Method Development & Selectivity Scouting
Kit 4104 is designed for systematic evaluation and optimization of chromatographic methods across HILIC and RP modes, particularly on standard analytical LC systems.
Column Selectivity Coverage
Diamond Hydride™ – HILIC‑style retention for highly polar analytes
Silica‑C™ – Normal‑phase / HILIC behavior for low‑water or non‑aqueous systems
Bidentate C18™ – Reversed‑phase retention for moderately polar to hydrophobic compounds
Bidentate C8™ – Reduced hydrophobicity for altered RP selectivity
UDC‑Cholesterol™ – Shape‑selective RP phase for aromatic and sterically distinct analytes
Together, these columns enable comprehensive selectivity mapping using a consistent TYPE‑C™ surface chemistry.
Why Choose the 4.6 mm × 100 mm Format
The 4.6 mm ID × 100 mm length format is commonly selected when greater resolving power than 50 mm scouting columns is needed, while retaining robust compatibility with standard instrumentation:
Improved resolution and peak capacity compared to short formats
Moderate run times suitable for method optimization
Standard analytical flow rates compatible with most LC systems
Well suited for UV, ELSD, CAD, and LC‑MS workflows
This format is ideal for refining methods after preliminary phase selection.
Key Advantages of Cogent TYPE‑C™ Technology
Across all five columns, the TYPE‑C™ silica hydride platform provides:
Faster equilibration than conventional bonded silica phases
Improved retention stability across aqueous and high‑organic conditions
Robust column lifetime in both HILIC and RP modes
Consistent behavior across different stationary chemistries, simplifying method comparison
These benefits significantly reduce development time and improve reproducibility.
System Requirements and Flow Rates
Typical flow range (4.6 mm ID): ~1.0–1.5 mL/min
Compatible with standard analytical HPLC and LC systems
No specialized low‑flow or UHPLC‑only hardware required
Final operating conditions should be optimized based on mobile‑phase composition, temperature, and system pressure limits.