Method Development Kit 2054, TYPE-C. Includes 5 Cogent HPLC Columns, 2.1mm x 50mm. Cogent TYPE-C Brand. 1 PK.
Additional Info:
The 40100‑05P‑2 Method Development Kit 2054 is a compact, application‑focused multi‑column kit designed for rapid method development and selectivity scouting in HILIC and reversed‑phase LC‑MS workflows. This kit provides an economical way to evaluate five complementary Cogent TYPE‑C™ stationary phases using a matched short‑length analytical format, ideal when speed, throughput, and solvent efficiency are priorities.
All columns in this kit are manufactured with Cogent TYPE‑C™ silica hydride technology, delivering fast equilibration, stable retention behavior, and robust lifetime performance across diverse mobile‑phase conditions commonly used in LC‑MS.
Kit Composition
This method‑development kit includes five analytical HPLC columns, each offering distinct selectivity while sharing identical physical specifications for direct comparison:
Shared specifications for all columns:
Internal Diameter: 2.1 mm
Length: 50 mm
Particle Size: 4 µm
Pore Size: 100 Å
Stationary Phase Platform: Cogent TYPE‑C™ silica hydride
The fully matched geometry ensures that observed differences during method scouting arise from stationary‑phase selectivity, not column dimensions or pressure effects.
Application Focus: Rapid Method Development & Selectivity Scouting
This kit is optimized for fast screening and method exploration, enabling systematic evaluation across HILIC and RP chemistries:
Column Selectivity Coverage
Diamond Hydride™ – HILIC‑style retention for highly polar analytes
Silica‑C™ – Normal‑phase / HILIC behavior for non‑aqueous and mixed‑mode separations
Bidentate C18™ – Reversed‑phase retention for moderately polar to hydrophobic compounds
Bidentate C8™ – Reduced hydrophobicity relative to C18 for altered RP selectivity
UDC‑Cholesterol™ – Shape‑selective RP phase for aromatic and sterically distinct molecules
This breadth of selectivity makes Kit 2054 particularly useful for early‑stage method development, scouting, and feasibility studies.
Why Choose the 2.1 mm × 50 mm Format
The 2.1 mm ID × 50 mm length configuration is designed for speed and efficiency:
Short run times and high throughput
Rapid equilibration between injections
Reduced solvent and sample consumption
Excellent compatibility with LC‑MS and LC‑MS/MS systems
This format is ideal when quickly identifying the most promising stationary phase before transitioning to longer columns for higher resolution.
Key Advantages of Cogent TYPE‑C™ Technology
Across all five columns, the TYPE‑C™ silica hydride platform provides:
Faster equilibration than traditional bonded phases
Improved retention stability under both high‑organic and aqueous conditions
Robust column lifetime across HILIC and RP modes
Consistent behavior across different chemistries, simplifying method comparison
These attributes substantially reduce method‑development time and variability.
System Requirements and Flow Rates
Typical flow range (2.1 mm ID): ~0.2–0.3 mL/min
Requires an LC or LC‑MS system capable of accurate and stable low‑flow delivery
Final conditions should be optimized based on mobile‑phase composition, temperature, and system pressure limits.