Method Development Kit 4054, TYPE-C. Includes 5 Cogent HPLC Columns, 4.6mm x 50mm. Cogent TYPE-C Brand. 1 PK.
Additional Info:
The 40100‑05P Method Development Kit 4054 is an analytical, high‑throughput method‑development and selectivity‑scouting kit built on Cogent TYPE‑C™ silica hydride technology. This kit provides an economical way to evaluate five complementary stationary phases commonly used in HILIC, reversed‑phase, and mixed‑mode LC methods, using a matched 4.6 mm × 50 mm analytical format.
All five columns share identical dimensions and particle properties, allowing chromatographers to directly compare stationary‑phase selectivity without interference from column geometry or system‑pressure differences.
Kit Composition
This method‑development kit includes five analytical HPLC columns, each offering distinct selectivity while sharing the same physical specifications:
Shared specifications for all columns:
Internal Diameter: 4.6 mm
Length: 50 mm
Particle Size: 4 µm
Pore Size: 100 Å
Stationary Phase Platform: Cogent TYPE‑C™ silica hydride
This standardized configuration ensures rapid and unbiased method scouting and selectivity screening.
Application Focus: Analytical Method Development & Scouting
Kit 4054 is optimized for rapid analytical evaluation across HILIC and RP chemistries, making it ideal for early‑stage development, feasibility testing, and robustness studies.
Column Selectivity Coverage
Diamond Hydride™ – HILIC‑style retention for highly polar analytes
Silica‑C™ – Normal‑phase / HILIC behavior for low‑water or non‑aqueous systems
Bidentate C18™ – Reversed‑phase retention for moderately polar to hydrophobic compounds
Bidentate C8™ – Reduced hydrophobicity vs C18 for altered RP selectivity
UDC‑Cholesterol™ – Shape‑selective RP phase for aromatic and sterically distinct analytes
Together, these phases allow comprehensive selectivity mapping using a consistent TYPE‑C™ surface chemistry.
Why Choose the 4.6 mm × 50 mm Format
The 4.6 mm ID × 50 mm length format is purpose‑built for speed and system compatibility:
Very short run times for rapid screening
Fast equilibration between injections
Standard analytical flow rates compatible with virtually all HPLC systems
Ideal for UV, ELSD, CAD, and LC‑MS method scouting
This format is commonly used when quickly identifying the most promising stationary phase prior to transitioning to longer columns for higher resolution.
Key Advantages of Cogent TYPE‑C™ Technology
Across all five columns, the TYPE‑C™ silica hydride platform delivers:
Faster equilibration than conventional bonded silica phases
Improved retention stability under both high‑organic and aqueous conditions
Robust column lifetime across HILIC and RP modes
Consistent behavior across different stationary chemistries, simplifying method comparison
These advantages significantly reduce development time and method variability.
System Requirements and Flow Rates
Typical flow range (4.6 mm ID): ~1.0–1.5 mL/min
Compatible with standard analytical HPLC and LC systems
No specialized low‑flow or UHPLC‑only hardware required
Final operating conditions should be optimized based on mobile‑phase composition, temperature, and system pressure limits.