Semi Prep HPLC Column, Diamond Hydride, 4 um, 100 A. 10 mm ID x 100 mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 70000‑SP100 Diamond Hydride Semi‑Preparative HPLC Column is designed for method scale‑up and purification workflows that require greater chromatographic resolution than shorter semi‑prep formats can provide. This column enables reliable isolation and collection of milligram‑level quantities of polar compounds while preserving the selectivity and retention characteristics established at the analytical scale.
Semi‑Preparative Use Case: Why 10 mm ID × 100 mm
The 10 mm internal diameter classifies this column as semi‑preparative, making it ideal for laboratories transitioning from analytical HPLC to purification without moving directly into full preparative hardware.
Compared to 50 mm semi‑prep columns, the 100 mm bed length provides:
Increased resolving power for closely eluting compounds
Higher peak capacity for complex sample matrices
Improved separation confidence during purification
Better alignment with analytical methods during scale‑up
This makes the 70000‑SP100 especially well suited for applications where resolution matters as much as loading capacity.
Diamond Hydride™ TYPE‑C Stationary Phase Advantages
Manufactured with Cogent TYPE‑C™ silica hydride technology, the Diamond Hydride stationary phase offers well‑established benefits for semi‑prep HILIC separations:
Stable and reproducible retention for polar analytes
Faster equilibration than conventional bonded‑silica HILIC phases
Robust column lifetime, even under high‑organic mobile‑phase conditions
This chemistry is widely used for purification of:
Peptides and peptide‑like compounds
Metabolites and highly polar small molecules
Analytes containing amine and basic functional groups
Other compounds requiring HILIC‑based isolation
Scaled Performance Without Radical Workflow Changes
The 70000‑SP100 allows laboratories to scale analytical methods logically and predictably, maintaining chromatographic behavior while enabling higher sample loads and fraction collection. It serves as an effective bridge between analytical method development and larger‑scale preparative purification.
System Requirements
Typical operating flow rates: ~3–8 mL/min
Requires a semi‑preparative HPLC system capable of:
Higher flow delivery
Larger injection volumes
Suitable detector configuration and fraction collection
Standard analytical HPLC systems are not suitable for 10 mm ID semi‑preparative columns.
Flow rates may be optimized further based on mobile‑phase viscosity, pressure limits, and purification goals.
Made‑to‑Order Columns & Pricing Information
These columns represent a significant investment and are often application‑specific. To ensure customers receive the correct column for their workflow:
Pricing and technical support is provided through our Customer Service Department via formal quotation. Use our online form to start the process or call customer service.
Columns are produced on order, based on customer requirements
Orders cannot be returned or canceled once placed