HPLC Column, UDC Cholesterol 2.o, 2.2um, 2.1mm ID x 150mm Length, 120A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 69269‑15P‑2 Cogent UDC‑Cholesterol 2.0 HPLC Column is engineered for maximum‑resolution separations using 2.2 µm TYPE‑C™ silica hydride particles modified with a cholesterol‑terminated ligand, enabling unique shape‑based selectivity.
This stationary phase consists of an 11‑carbon linker bonded directly to silica hydride with a cholesterol group at the terminus, forming structured interaction sites that act as molecular “slots.” These sites allow analytes to be separated based on shape, steric orientation, and molecular geometry, not just hydrophobicity.
Key structural advantages include:
Direct Si–C bonding (no end‑capping required)
Reduced secondary interactions
Enhanced stability and reproducibility
This column supports multiple chromatographic modes:
Reversed Phase
Normal Phase
HILIC
150 mm Length – Maximum Resolution & Selectivity
Highest resolving power in the UDC 2.1 mm column range
Ideal for complex mixtures and difficult separations
Maximizes isomer separation and peak capacity
Preferred for LC‑MS workflows requiring highest resolution
Longer run times compared to 50 mm and 100 mm options
Flow Rate & System Requirements (2.1 mm ID)
Requires precision UHPLC low‑flow capability
Typical flow rate range: ~0.2–0.4 mL/min
Optimal starting range: ~0.18–0.28 mL/min for maximum resolution
Requires:
UHPLC system capable of stable, accurate low‑flow delivery
Low‑dispersion plumbing and flow cell
High precision injection system
⚠️ Standard HPLC systems must be capable of reliable low‑flow operation to achieve expected performance.
Why Choose UDC‑Cholesterol™
Shape & Isomer Selectivity
Separates analytes based on 3D structure and steric fit
Exceptional for isomer and structurally similar compound separation
Strong Hydrophobic Interaction
Highly hydrophobic phase
Effective retention of lipids and difficult compounds
Tunable Selectivity
Selectivity can be adjusted by:
Temperature changes
Mobile phase composition
Enables fine control of separation profile
Cogent TYPE‑C™ Technology Advantages
Silica Hydride Surface
Direct Si–C bond eliminates end‑capping requirement
Reduced silanol activity
Improved peak shape for basic and polar compounds
Faster Equilibration
Faster equilibration vs traditional RP and HILIC columns
Improved throughput despite longer column length
Robust Column Lifetime
Resistant to hydrolysis
Stable across multiple solvent systems
Long operational durability
Typical Applications
Steroid and structural isomer analysis
Lipid separations
Peptides, proteins, and oligonucleotides
Complex biological or pharmaceutical samples
LC‑MS methods requiring maximum resolution
Compounds poorly resolved on C18 or C8