Catalog Number - 69269-10P-2

The 69269‑10P‑2 Cogent UDC‑Cholesterol 2.0 HPLC Column delivers advanced shape‑based selectivity using 2.2 µm TYPE‑C™ silica hydride particles modified with a cholesterol‑terminated ligand.

This stationary phase consists of an 11‑carbon chain bonded directly to silica hydride with a cholesterol group at the terminus, creating structured interaction regions that act as molecular “slots.” These allow separation based on molecular shape, steric fit, and geometry, not just hydrophobic interaction—providing selectivity beyond traditional reversed‑phase columns.

Unlike conventional silica:

• Direct Si–C bonding eliminates the need for end‑capping

• Reduced silanol activity and secondary interactions

• Improved reproducibility and stability

This column is compatible with multiple chromatographic modes:

• Reversed Phase

• Normal Phase

• HILIC

100 mm Length – Increased Resolution for LC‑MS

• Higher resolving power vs 50 mm columns

• Improved isomer separation and peak capacity

• Ideal for LC‑MS methods requiring enhanced resolution

• Balanced trade‑off between speed and separation quality

This length is one of the most commonly selected formats for UHPLC / LC‑MS workflows when additional resolution is required without excessive run time.

Flow Rate & System Requirements (2.1 mm ID)

Requires precise low‑flow UHPLC capability

• Typical flow rate range: ~0.2–0.4 mL/min

• Optimal starting range: ~0.2–0.3 mL/min for improved resolution

• Requires:

  • UHPLC system capable of stable low‑flow delivery

  • Low‑volume flow cell detector

  • Precise injection system for low‑volume samples

⚠️ Standard HPLC systems must be capable of reliable low‑flow operation to achieve proper performance.

Why Choose UDC‑Cholesterol™

Shape & Isomer Selectivity

• Separates analytes based on 3D structure and steric interactions

• Ideal for isomers and structurally similar compounds

High Hydrophobicity

• Strong retention of lipophilic analytes

• Effective for difficult‑to‑retain compounds

Tunable Selectivity

• Selectivity can be modified through:

  • Temperature adjustments

  • Mobile phase composition changes

• Provides flexibility for challenging separations

Cogent TYPE‑C™ Technology Advantages

Silica Hydride Surface

• Direct Si–C bond (no end‑capping required)

• Reduced silanol interactions

• Improved peak shape for basic and polar compounds

Faster Equilibration

• Rapid re‑equilibration vs conventional RP and HILIC columns

• Increased throughput

Robust Column Lifetime

• Resistant to hydrolysis

• Stable across multiple solvent systems

• Long operational durability

Typical Applications

• Steroidal compound separations

• Structural isomer analysis

• Lipids and highly hydrophobic compounds

• Peptides, proteins, and oligonucleotides

• LC‑MS workflows requiring increased resolution

• Compounds difficult to separate using C18 or C8

This UDC Cholesterol™ HPLC is made with Cogent TYPE-C™ Silica, and