Catalog Number - 69069-SP50

The 69069‑SP50 Cogent UDC‑Cholesterol Semi‑Preparative HPLC Column is designed for high‑load separations and compound purification, utilizing 4 µm TYPE‑C™ silica hydride particles modified with a cholesterol‑terminated ligand to deliver unique shape‑based selectivity.

This stationary phase features an 11‑carbon chain directly bonded to silica hydride with a cholesterol moiety at the terminus, forming structured interaction sites that act as molecular “slots.” These sites allow separation based on molecular geometry and steric interactions, not just hydrophobicity—making it ideal for difficult isomer separations and complex mixtures.

Unlike traditional reversed‑phase columns:

• Direct Si–C bonding eliminates the need for end‑capping

• Reduced silanol activity and secondary interactions

• Improved reproducibility and durability

Compatible with multiple chromatographic modes:

• Reversed Phase

• Normal Phase

• HILIC

Semi‑Preparative Use‑Case Framework

50 mm Length – Fast Purification & Throughput

• Optimized for rapid purification cycles

• Lower backpressure at higher flow rates

• Ideal for higher throughput fraction collection

• Best suited when speed is prioritized over maximum resolution

Flow Rate & System Requirements (10 mm ID)

Semi‑prep columns require specialized HPLC systems

• Typical flow rate range: ~4 – 12 mL/min

• Optimal starting range: ~5 – 8 mL/min

• Requires:

  • Semi‑preparative HPLC system with high‑flow capability

  • Appropriately sized pump heads and tubing

  • Fraction collection system for purified analytes

⚠️ Standard analytical HPLC systems are not sufficient for this column size.

Flow rates should be optimized based on:

• Sample load

• Desired resolution vs speed

• Solvent viscosity

• System pressure limits

Why Choose UDC‑Cholesterol™ for Semi‑Prep

Shape‑Selective Purification

• Separates compounds by 3D structure and steric fit

• Enables isolation of closely related isomers

Strong Hydrophobic Interaction

• Effective retention of lipids and hydrophobic compounds

• Suitable for difficult analytes

Flexible Selectivity Control

• Adjust separation using:

  • Temperature

  • Mobile phase composition

• Enables optimized purification methods

Cogent TYPE‑C™ Technology Advantages

Silica Hydride Surface

• Direct Si–C bonding (no end‑capping)

• Reduced secondary interactions

• Improved peak shape and recovery

Faster Equilibration

• Shorter cycle times

• Increased purification throughput

Robust Column Lifetime

• Resistant to hydrolysis

• Stable under high‑load conditions

  Extended operational durability

Typical Applications

• Semi‑preparative purification of steroidal compounds

• Isolation of structural isomers

• Lipids and hydrophobic analytes

• Peptides, proteins, and oligonucleotides

• Compound collection from analytical method scale‑up

• Applications where C18/C8 selectivity is insufficient

Important Usage Notes

• Requires semi‑preparative HPLC system

• Designed for higher flow rates and sample loading

• Ideal for fast purification workflows

• Optimize temperature and solvent to control selectivity

Made‑to‑Order & Pricing Information

This is a custom, made‑to‑order item.

• Orders cannot be canceled once placed

• Custom items are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification:

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.