Semi Prep HPLC Column, UDC Cholesterol, 4um, 100A. 10mm ID x 250mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 69069‑SP250 Cogent UDC‑Cholesterol Semi‑Preparative HPLC Column is engineered for maximum separation performance in purification workflows, utilizing 4 µm TYPE‑C™ silica hydride particles modified with a cholesterol‑terminated ligand.
This stationary phase incorporates an 11‑carbon chain directly bonded to silica hydride with a terminal cholesterol group, forming structured interaction regions that act as molecular “slots.” These enable separation based on molecular geometry, steric orientation, and shape, offering selectivity beyond conventional reversed‑phase columns.
Key design advantages:
Direct Si–C bonding (no end‑capping required)
Reduced silanol activity and secondary interactions
Improved reproducibility and robustness
This column supports multiple chromatographic modes:
Reversed Phase
Normal Phase
HILIC
Semi‑Preparative Use‑Case Framework
250 mm Length – Maximum Separation & Peak Capacity
Highest resolving power within the 10 mm semi‑prep range
Maximizes peak capacity for complex mixtures
Ideal for final purification and high‑purity fractions
Best suited for closely related compounds and isomers
Longer run times vs 50 mm, 100 mm, and 150 mm columns
Flow Rate & System Requirements (10 mm ID)
Semi‑prep columns require specialized HPLC systems
Typical flow rate range: ~4 – 12 mL/min
Optimal starting range: ~3.5 – 6 mL/min for maximum resolution
Requires:
Semi‑preparative HPLC system with high‑flow capability
Heavy‑duty pump heads and tubing
Fraction collection system for purification
⚠️ Standard analytical HPLC systems are not suitable for this column size.
Flow optimization depends on:
Desired resolution vs throughput
Sample load
Solvent viscosity
System pressure limits
Why Choose UDC‑Cholesterol™ for Semi‑Prep
Shape‑Selective Purification
Separates compounds based on 3D structure and steric fit
Ideal for isolating closely related isomers
Strong Hydrophobic Interaction
Highly hydrophobic phase
Effective for lipids and difficult analytes
Tunable Selectivity
Adjust separation using:
Temperature changes
Mobile phase composition
Enables precise purification control
Cogent TYPE‑C™ Technology Advantages
Silica Hydride Surface
Direct Si–C bonding eliminates end‑capping
Reduced secondary interactions
Improved peak shape and recovery
Faster Equilibration
Shorter cycle times
Increased purification throughput
Robust Column Lifetime
Resistant to hydrolysis
Stable under high‑load conditions
Long operational durability
Typical Applications
Semi‑preparative purification of steroidal compounds
Isolation of structural isomers
Lipids and highly hydrophobic analytes
Peptides, proteins, and oligonucleotides
Complex multi‑component mixtures
Applications where C18/C8 phases lack sufficient selectivity
Important Usage Notes
Requires semi‑preparative HPLC system
Designed for high‑flow, high‑load purification workflows
Ideal for maximum‑resolution separations and final product isolation
Optimize temperature and mobile phase to control selectivity
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable