Catalog Number - 69069-SP150

The 69069‑SP150 Cogent UDC‑Cholesterol Semi‑Preparative HPLC Column is designed for maximum‑resolution purification and high‑load separations, using 4 µm TYPE‑C™ silica hydride particles modified with a cholesterol‑terminated ligand to deliver unique shape‑based selectivity.

This stationary phase employs an 11‑carbon chain bonded directly to silica hydride with a terminal cholesterol group, forming structured interaction regions that behave as molecular “slots.” These enable separations based on molecular geometry and steric fit, rather than hydrophobic interaction alone—making this column highly effective for isomeric and closely related compounds.

Key design features:

• Direct Si–C bonding (no end‑capping required)

• Reduced silanol activity and secondary interactions

• Enhanced durability and reproducibility

Supports multiple chromatographic modes:

• Reversed Phase

• Normal Phase

• HILIC

Semi‑Preparative Use‑Case Framework

150 mm Length – Maximum Resolution Purification

• High resolving power for complex mixtures

• Improves fraction purity and separation of closely related analytes

• Ideal for final purification workflows

• Longer run times compared to 50 mm and 100 mm columns

Flow Rate & System Requirements (10 mm ID)

Semi‑prep columns require specialized HPLC systems

• Typical flow rate range: ~4 – 12 mL/min

• Optimal starting range: ~4 – 7 mL/min for maximum resolution

• Requires:

  • Semi‑preparative HPLC system with high‑flow capability

  • Appropriate pump heads and tubing

  • Fraction collection system

⚠️ Standard analytical HPLC systems are not suitable for this column size.

Optimize flow based on:

• Sample loading

• Desired purity and resolution

• Solvent viscosity

• System pressure limits

Why Choose UDC‑Cholesterol™ for Semi‑Prep

Shape‑Selective Purification

• Separates analytes based on 3D structure and steric interaction

• Excellent for isolating closely related isomers

Strong Hydrophobic Interaction

• Highly hydrophobic phase for lipids and difficult compounds

• Enables retention of challenging analytes

Tunable Selectivity

• Adjust separation using:

  • Temperature changes

  • Mobile phase composition

• Enables method optimization for difficult separations

Cogent TYPE‑C™ Technology Advantages

Silica Hydride Surface

• Direct Si–C bonding (no end‑capping)

• Reduced secondary interactions

• Improved peak shape and recovery

Faster Equilibration

• Reduced run‑to‑run cycle time

• Increased purification throughput

Robust Column Lifetime

• Resistant to hydrolysis

• Stable under high‑load purification conditions

• Long operational durability

Typical Applications

• Semi‑preparative purification of steroidal compounds

• Isolation of structural isomers

• Lipids and hydrophobic analytes

• Peptides, proteins, and oligonucleotides

• Analytical method scale‑up

• Applications where C18/C8 phases lack selectivity

Important Usage Notes

• Requires semi‑preparative HPLC system

• Designed for higher flow rates and sample loading

• Ideal for maximum‑resolution purification workflows

• Optimize temperature and solvent composition to control selectivity

Made‑to‑Order & Pricing Information

This is a custom, made‑to‑order item.

• Orders cannot be canceled once placed

• Custom items are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification:

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.