Semi Prep HPLC Column, UDC Cholesterol, 4um, 100A. 10mm ID x 100mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 69069‑SP100 Cogent UDC‑Cholesterol Semi‑Preparative HPLC Column is designed for high‑load purification with enhanced resolution, using 4 µm TYPE‑C™ silica hydride particles modified with a cholesterol‑terminated ligand.
This stationary phase incorporates an 11‑carbon chain directly bonded to silica hydride with a terminal cholesterol group, creating structured interaction regions that act as molecular “slots.” These enable separations based on molecular geometry and steric fit, making this column highly effective for isomer separation and complex mixtures.
Key advantages:
Direct Si–C bonding (no end‑capping required)
Reduced silanol interactions
Improved durability and reproducibility
This column supports multiple chromatographic modes:
Reversed Phase
Normal Phase
HILIC
Semi‑Preparative Use‑Case Framework
100 mm Length – Increased Resolution Purification
Higher resolving power compared to 50 mm columns
Improved fraction purity and separation of closely related compounds
Balanced performance between speed and resolution
Ideal for analytical method scale‑up to purification workflows
Flow Rate & System Requirements (10 mm ID)
Semi‑prep columns require specialized HPLC systems
Typical flow rate range: ~4 – 12 mL/min
Optimal starting range: ~4 – 8 mL/min for improved resolution
Requires:
Semi‑preparative HPLC system with high‑flow capability
Appropriate pump heads and tubing
Fraction collection system
⚠️ Standard analytical HPLC systems are not suitable for this column size.
Flow should be optimized based on:
Sample load
Resolution requirements
Solvent viscosity
System pressure limits
Why Choose UDC‑Cholesterol™ for Semi‑Prep
Shape‑Selective Purification
Separates compounds based on 3D structure and steric interactions
Ideal for isolating closely related isomers
Strong Hydrophobic Interaction
Effective retention of lipids and hydrophobic compounds
Suitable for difficult analytes
Tunable Selectivity
Adjust separation with:
Temperature changes
Mobile phase composition
Enables optimized purification strategies
Cogent TYPE‑C™ Technology Advantages
Silica Hydride Surface
Direct Si–C bonding (no end‑capping)
Reduced secondary interactions
Improved peak shape and recovery
Faster Equilibration
Shorter cycle times
Increased purification throughput
Robust Column Lifetime
Resistant to hydrolysis
Stable under high‑load conditions
Long operational durability
Typical Applications
Semi‑prep purification of steroidal compounds
Isolation of structural isomers
Lipids and hydrophobic analytes
Peptides, proteins, and oligonucleotides
Method scale‑up from analytical to purification
Applications where C18/C8 selectivity is insufficient
Important Usage Notes
Requires semi‑preparative HPLC system
Designed for higher flow rates and larger injection volumes
Ideal for balanced purification workflows (resolution + throughput)
Optimize temperature and solvent to control selectivity
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable