Catalog Number - 69069-P30-50

The 69069‑P30‑50 Cogent UDC‑Cholesterol Preparative HPLC Column is designed for high‑capacity purification at large scale, utilizing 4 µm TYPE‑C™ silica hydride particles modified with a cholesterol‑terminated ligand to deliver unique shape‑based selectivity.

This stationary phase features an 11‑carbon chain directly bonded to silica hydride with a terminal cholesterol group, forming structured interaction regions that act as molecular “slots.” These enable separations based on molecular geometry, steric fit, and shape, beyond hydrophobic interaction—making the column highly effective for closely related compounds and isomers.

Key advantages:

• Direct Si–C bonding (no end‑capping required)

• Reduced silanol activity and secondary interactions

• Enhanced reproducibility and durability under high‑load preparative conditions

Supports multiple chromatographic modes:

• Reversed Phase

• Normal Phase

• HILIC

Preparative Column Use‑Case Framework

50 mm Length – Fast, High‑Load Purification

• Optimized for rapid purification cycles at large scale

• Lower backpressure despite high flow rates

• Ideal for high‑throughput fraction collection

• Preferred when speed and throughput outweigh maximum resolution

Flow Rate & System Requirements (30 mm ID)

Large‑scale preparative columns require specialized high‑flow systems

• Typical flow rate range: ~40 – 120 mL/min

• Optimal starting range: ~60 – 90 mL/min

• Requires:

  • Preparative HPLC system with high‑flow capability

  • High‑capacity pump heads, tubing, and fittings

  • Fraction collection system

  • Proper solvent delivery infrastructure

⚠️ Standard analytical HPLC systems cannot support this column size.

Flow rates must be optimized based on:

• Sample load and injection volume

• Resolution vs throughput requirements

• Solvent viscosity

• System pressure limits

Why Choose UDC‑Cholesterol™ for Preparative Work

Shape‑Selective Purification

• Separates analytes based on 3D structure and steric interaction

• Enables isolation of closely related isomers

Strong Hydrophobic Interaction

• Highly hydrophobic phase

• Effective for lipids and difficult compounds

Tunable Selectivity

• Adjust separation using:

  • Temperature changes

  • Mobile phase composition

• Enables precise purification control

Cogent TYPE‑C™ Technology Advantages

Silica Hydride Surface

• Direct Si–C bonding (no end‑capping required)

• Reduced secondary interactions

• Improved peak shape and compound recovery

Faster Equilibration

• Reduced cycle times

• Increased purification throughput

Robust Column Lifetime

• Resistant to hydrolysis

• Stable under high‑load, high‑flow conditions

• Long operational durability

Typical Applications

• Large‑scale purification of steroidal compounds

• Isolation of structural isomers

• Lipids and hydrophobic analytes

• Peptides, proteins, and oligonucleotides

• High‑throughput preparative workflows

• Scale‑up from analytical and semi‑prep methods

Important Usage Notes

• Requires a full preparative HPLC system

• Designed for high‑flow, high‑load purification workflows

• Best for fast purification at large scale

• Optimize temperature and mobile phase to control selectivity

Made‑to‑Order & Pricing Information

This is a custom, made‑to‑order item.

• Orders cannot be canceled once placed

• Custom items are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification:

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.