Prep HPLC Column, UDC Cholesterol, 4um, 100A. 30mm ID x 250mm Length, Preparative. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 69069‑P30‑250 Cogent UDC‑Cholesterol Preparative HPLC Column is engineered for maximum separation performance at the largest purification scale, utilizing 4 µm TYPE‑C™ silica hydride particles modified with a cholesterol‑terminated ligand to deliver unique shape‑based selectivity.
This stationary phase features an 11‑carbon chain directly bonded to silica hydride with a terminal cholesterol group, forming structured interaction regions that act as molecular “slots.” These allow compounds to be separated based on molecular geometry, steric orientation, and shape, in addition to hydrophobic interaction—making it highly effective for closely related compounds, positional isomers, and complex mixtures.
Key advantages:
Direct Si–C bonding (no end‑capping required)
Reduced silanol activity and secondary interactions
Enhanced durability and reproducibility under high‑load conditions
Supports multiple chromatographic modes:
Reversed Phase
Normal Phase
HILIC
Preparative Column Use‑Case Framework
250 mm Length – Maximum Separation & Peak Capacity
Highest resolving power in the 30 mm preparative column range
Maximizes peak capacity for highly complex mixtures
Ideal for final purification and ultra‑high‑purity fraction collection
Best suited for closely related isomers and difficult separations
Longest run times compared to 50 mm, 100 mm, and 150 mm formats
Flow Rate & System Requirements (30 mm ID)
Large‑scale preparative columns require specialized high‑flow systems
Typical flow rate range: ~40 – 120 mL/min
Optimal starting range: ~35 – 70 mL/min for maximum resolution
Requires:
Preparative HPLC system with high‑flow capability
High‑capacity pump heads, tubing, and fittings
Fraction collection system
Proper solvent handling infrastructure
⚠️ Standard analytical HPLC systems are not suitable for this column size.
Flow optimization depends on:
Sample load and injection volume
Desired resolution vs throughput
Solvent viscosity
System pressure limits
Why Choose UDC‑Cholesterol™ for Preparative Work
Shape‑Selective Purification
Separates analytes based on 3D structure and steric interaction
Ideal for isolating closely related isomers
Strong Hydrophobic Interaction
Highly hydrophobic stationary phase
Effective retention for lipids and difficult compounds
Tunable Selectivity
Adjust separation conditions using:
Temperature changes
Mobile phase composition
Enables precise purification control
Cogent TYPE‑C™ Technology Advantages
Silica Hydride Surface
Direct Si–C bonding eliminates end‑capping requirement
Reduced secondary interactions
Improved peak shape and recovery
Faster Equilibration
Shorter cycle times
Increased purification throughput
Robust Column Lifetime
Resistant to hydrolysis
Stable under high‑flow, high‑load conditions
Long operational durability
Typical Applications
Large‑scale purification of steroidal compounds
Isolation of structural isomers
Lipids and highly hydrophobic analytes
Peptides, proteins, and oligonucleotides
Complex multi‑component mixtures
Final purification where maximum separation is required
Applications where C18/C8 phases lack sufficient selectivity
Important Usage Notes
Requires a full preparative HPLC system
Designed for high‑flow, high‑load purification workflows
Ideal for maximum separation and highest purity isolation
Optimize temperature and mobile phase to fine‑tune selectivity
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable