Prep HPLC Column, UDC Cholesterol, 4um, 100A. 30mm ID x 150mm Length, Preparative. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 69069‑P30‑150 Cogent UDC‑Cholesterol Preparative HPLC Column is engineered for maximum‑resolution, large‑scale purification, utilizing 4 µm TYPE‑C™ silica hydride particles modified with a cholesterol‑terminated ligand to deliver unique shape‑based selectivity.
This stationary phase features an 11‑carbon chain directly bonded to silica hydride with a terminal cholesterol group, forming structured interaction regions that act as molecular “slots.” These enable separations based on molecular geometry, steric orientation, and shape, rather than hydrophobic interaction alone—making it highly effective for closely related isomers and complex mixtures.
Key advantages:
Direct Si–C bonding (no end‑capping required)
Reduced silanol activity and secondary interactions
Enhanced durability and reproducibility under preparative conditions
Supports multiple chromatographic modes:
Reversed Phase
Normal Phase
HILIC
Preparative Column Use‑Case Framework
150 mm Length – Maximum Resolution & Purity
High resolving power for complex mixtures
Optimized for maximum purity in fraction collection
Ideal for final purification workflows
Improves separation of closely related compounds and isomers
Longer run times vs 50 mm and 100 mm options
Flow Rate & System Requirements (30 mm ID)
Large‑scale preparative columns require specialized high‑flow systems
Typical flow rate range: ~40 – 120 mL/min
Optimal starting range: ~35 – 70 mL/min for maximum resolution
Requires:
Preparative HPLC system with high‑flow capability
High‑capacity pump heads, tubing, and fittings
Fraction collection system
⚠️ Standard analytical HPLC systems are not compatible with this column size.
Flow optimization depends on:
Sample loading and injection volume
Resolution vs throughput requirements
Solvent viscosity
System pressure limits
Why Choose UDC‑Cholesterol™ for Preparative Work
Shape‑Selective Purification
Separates analytes based on 3D structure and steric interaction
Ideal for isolating closely related isomers
Strong Hydrophobic Interaction
Highly hydrophobic stationary phase
Effective for lipids and difficult compounds
Tunable Selectivity
Adjust separation using:
Temperature changes
Mobile phase composition
Enables precise purification optimization
Cogent TYPE‑C™ Technology Advantages
Silica Hydride Surface
Direct Si–C bonding (no end‑capping required)
Reduced secondary interactions
Improved peak shape and recovery
Faster Equilibration
Shorter cycle times
Increased purification throughput
Robust Column Lifetime
Resistant to hydrolysis
Stable under high‑flow, high‑load conditions
Long operational durability
Typical Applications
Large‑scale purification of steroidal compounds
Isolation of structural isomers
Lipids and hydrophobic analytes
Peptides, proteins, and oligonucleotides
Complex multi‑component mixtures
Applications where C18/C8 columns lack sufficient selectivity
Important Usage Notes
Requires a full preparative HPLC system
Designed for high‑flow, high‑load purification workflows
Ideal for maximum‑resolution and high‑purity isolation
Optimize temperature and mobile phase to fine‑tune selectivity
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable