Prep HPLC Column, UDC Cholesterol, 4um, 100A. 30mm ID x 100mm Length, Preparative. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 69069‑P30‑100 Cogent UDC‑Cholesterol Preparative HPLC Column is designed for high‑capacity purification with enhanced resolution, using 4 µm TYPE‑C™ silica hydride particles modified with a cholesterol‑terminated ligand to deliver unique shape‑based selectivity.
This stationary phase features an 11‑carbon chain directly bonded to silica hydride with a terminal cholesterol group, forming structured interaction regions that act as molecular “slots.” These interactions allow analytes to be separated based on molecular geometry, steric fit, and shape, in addition to hydrophobic interaction—making it especially effective for closely related compounds and isomers.
Key advantages:
Direct Si–C bonding (no end‑capping required)
Reduced silanol activity and secondary interactions
Enhanced durability and reproducibility under preparative conditions
Supports multiple chromatographic modes:
Reversed Phase
Normal Phase
HILIC
Preparative Column Use‑Case Framework
100 mm Length – Increased Resolution Purification
Higher resolving power than 50 mm columns
Improved fraction purity and separation of closely related compounds
Balanced performance between throughput and resolution
Ideal for analytical‑to‑preparative method scale‑up
Flow Rate & System Requirements (30 mm ID)
Large‑scale preparative columns require specialized high‑flow systems
Typical flow rate range: ~40 – 120 mL/min
Optimal starting range: ~40 – 80 mL/min for enhanced resolution
Requires:
Preparative HPLC system with high‑flow capability
High‑capacity pump heads, tubing, and fittings
Fraction collection system
⚠️ Standard analytical HPLC systems are not suitable for this column size.
Flow rates should be optimized based on:
Sample load and injection volume
Desired resolution vs throughput
Mobile phase viscosity
System pressure limitations
Why Choose UDC‑Cholesterol™ for Preparative Work
Shape‑Selective Purification
Separates analytes based on 3D structure and steric interaction
Ideal for isolating closely related isomers
Strong Hydrophobic Interaction
Highly hydrophobic phase
Effective retention of lipids and difficult compounds
Tunable Selectivity
Optimize separations by adjusting:
Temperature
Mobile phase composition
Enables precise purification control
Cogent TYPE‑C™ Technology Advantages
Silica Hydride Surface
Direct Si–C bonding (no end‑capping required)
Reduced secondary interactions
Improved peak shape and recovery
Faster Equilibration
Shorter cycle times
Increased purification throughput
Robust Column Lifetime
Resistant to hydrolysis
Stable under high‑load, high‑flow conditions
Long operational durability
Typical Applications
Preparative purification of steroidal compounds
Isolation of structural isomers
Lipids and hydrophobic analytes
Peptides, proteins, and oligonucleotides
Analytical‑to‑prep method scale‑up
Applications where C18/C8 phases lack selectivity
Important Usage Notes
Requires a preparative HPLC system
Designed for high‑flow, high‑load purification workflows
Ideal for balanced purification (resolution + throughput)
Optimize temperature and mobile phase to control selectivity
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable