Prep HPLC Column, UDC Cholesterol, 4um, 100A. 21.2mm ID x 50mm Length, Preparative. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 69069‑P21‑50 Cogent UDC‑Cholesterol Preparative HPLC Column is designed for high‑capacity purification and preparative separations, utilizing 4 µm TYPE‑C™ silica hydride particles modified with a cholesterol‑terminated ligand to deliver unique shape‑based selectivity.
This stationary phase incorporates an 11‑carbon chain directly bonded to silica hydride with a terminal cholesterol group, forming structured interaction regions that act as molecular “slots.” These sites enable separation based on molecular geometry, steric interaction, and shape, not just hydrophobicity—making it especially effective for isomers and complex mixtures.
Key advantages:
Direct Si–C bonding (no end‑capping required)
Reduced silanol activity and secondary interactions
Improved reproducibility and durability under prep conditions
Supports multiple chromatographic modes:
Reversed Phase
Normal Phase
HILIC
Preparative Column Use‑Case Framework
50 mm Length – Fast Purification & Throughput
Optimized for rapid purification cycles
Lower backpressure at high flow rates
Ideal for high‑throughput fraction collection
Best suited when speed is prioritized over maximum resolution
Flow Rate & System Requirements (21.2 mm ID)
Preparative columns require specialized HPLC systems
Typical flow rate range: ~20 – 60 mL/min
Optimal starting range: ~25 – 45 mL/min
Requires:
Preparative HPLC system with high‑flow capability
High‑capacity pump heads and tubing
Fraction collection system
⚠️ Standard analytical HPLC systems are not suitable for this column size.
Flow should be optimized based on:
Sample load and injection volume
Desired resolution vs throughput
Solvent viscosity
System pressure limits
Why Choose UDC‑Cholesterol™ for Preparative Work
Shape‑Selective Purification
Separates compounds based on 3D structure and steric fit
Enables isolation of closely related isomers
Strong Hydrophobic Interaction
Highly hydrophobic stationary phase
Effective for lipids and difficult analytes
Tunable Selectivity
Adjust selectivity using:
Temperature changes
Mobile phase composition
Enables optimized purification outcomes
Cogent TYPE‑C™ Technology Advantages
Silica Hydride Surface
Direct Si–C bonding (no end‑capping required)
Reduced secondary interactions
Improved peak shape and recovery
Faster Equilibration
Shorter cycle times
Increased purification throughput
Robust Column Lifetime
Resistant to hydrolysis
Stable under high‑load preparative conditions
Long operational durability
Typical Applications
Preparative purification of steroidal compounds
Isolation of structural isomers
Lipids and hydrophobic analytes
Peptides, proteins, and oligonucleotides
Scale‑up from analytical to preparative workflows
Applications where C18/C8 phases lack sufficient selectivity
Important Usage Notes
Requires preparative HPLC system
Designed for high‑flow, high‑load purification workflows
Ideal for fast purification with high throughput
Optimize temperature and mobile phase to control selectivity
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable