Prep HPLC Column, UDC Cholesterol, 4um, 100A. 21.2mm ID x 250mm Length, Preparative. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 69069‑P21‑250 Cogent UDC‑Cholesterol Preparative HPLC Column is engineered for maximum separation and peak capacity, utilizing 4 µm TYPE‑C™ silica hydride particles modified with a cholesterol‑terminated ligand to deliver unique shape‑based selectivity.
This stationary phase uses an 11‑carbon chain directly bonded to silica hydride with a terminal cholesterol moiety, forming structured interaction regions that act as molecular “slots.” These interactions allow compounds to be separated based on molecular geometry, steric orientation, and shape, not just hydrophobicity—making this column highly effective for closely related compounds and difficult isomer separations.
Key advantages:
Direct Si–C bonding eliminates the need for end‑capping
Reduced silanol activity and secondary interactions
Improved reproducibility and durability under high‑load conditions
Supports multiple chromatographic modes:
Reversed Phase
Normal Phase
HILIC
Preparative Column Use‑Case Framework
250 mm Length – Maximum Separation & Peak Capacity
Highest resolving power in the 21.2 mm preparative column range
Maximizes peak capacity for highly complex mixtures
Ideal for final purification and high‑purity fraction collection
Best suited for closely related isomers and structurally similar compounds
Longer run times vs 50 mm, 100 mm, and 150 mm formats
Flow Rate & System Requirements (21.2 mm ID)
Preparative columns require specialized HPLC systems
Typical flow rate range: ~20 – 60 mL/min
Optimal starting range: ~18 – 40 mL/min for maximum resolution
Requires:
Preparative HPLC system with high‑flow capability
High‑capacity pump heads and tubing
Fraction collection system
⚠️ Standard analytical HPLC systems are not suitable for this column size.
Flow optimization should consider:
Sample load and injection volume
Resolution vs throughput requirements
Solvent viscosity
System pressure limits
Why Choose UDC‑Cholesterol™ for Preparative Work
Shape‑Selective Purification
Separates compounds based on 3D structure and steric fit
Ideal for isolating closely related isomers
Strong Hydrophobic Interaction
Highly hydrophobic stationary phase
Effective retention for lipids and difficult analytes
Tunable Selectivity
Adjust separation using:
Temperature changes
Mobile phase composition
Enables precise purification control
Cogent TYPE‑C™ Technology Advantages
Silica Hydride Surface
Direct Si–C bonding (no end‑capping required)
Reduced secondary interactions
Improved peak shape and recovery
Faster Equilibration
Shorter cycle times
Increased purification throughput
Robust Column Lifetime
Resistant to hydrolysis
Stable under high‑load preparative conditions
Long operational durability
Typical Applications
Preparative purification of steroidal compounds
Isolation of structural isomers
Lipids and hydrophobic analytes
Peptides, proteins, and oligonucleotides
Complex multi‑component mixtures
Applications where C18 or C8 phases lack sufficient selectivity
Important Usage Notes
Requires a preparative HPLC system
Designed for high‑flow, high‑load purification workflows
Ideal for maximum‑resolution, high‑purity separations
Optimize temperature and mobile phase to fine‑tune selectivity
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable