Catalog Number - 69069-P21-150

The 69069‑P21‑150 Cogent UDC‑Cholesterol Preparative HPLC Column is designed for maximum‑resolution purification, utilizing 4 µm TYPE‑C™ silica hydride particles functionalized with a cholesterol‑terminated ligand to deliver unique shape‑based selectivity.

This stationary phase features an 11‑carbon chain directly bonded to silica hydride with a cholesterol moiety at the terminus, creating structured interaction regions that act as molecular “slots.” These allow separations based on molecular geometry, steric orientation, and shape, rather than hydrophobic interaction alone—making this column highly effective for resolving isomers and closely related compounds.

Key structural advantages:

• Direct Si–C bonding (no end‑capping required)

• Reduced silanol activity and secondary interactions

• Improved reproducibility and robustness under preparative conditions

This column supports multiple chromatographic modes:

• Reversed Phase

• Normal Phase

• HILIC

Preparative Column Use‑Case Framework

150 mm Length – Maximum Resolution Purification

• High resolving power for complex mixtures

• Improves fraction purity and separation of closely related analytes

• Ideal for final purification workflows and high‑purity isolation

• Longer run times compared to 50 mm and 100 mm columns

Flow Rate & System Requirements (21.2 mm ID)

Preparative columns require specialized HPLC systems

• Typical flow rate range: ~20 – 60 mL/min

• Optimal starting range: ~20 – 40 mL/min for maximum resolution

• Requires:

  • Preparative HPLC system with high‑flow capability

  • High‑capacity pump heads and tubing

  • Fraction collection system

⚠️ Standard analytical HPLC systems are not suitable for this column size.

Flow optimization depends on:

• Sample load and injection volume

• Desired resolution vs throughput

• Solvent viscosity

• System pressure limits

Why Choose UDC‑Cholesterol™ for Preparative Work

Shape‑Selective Purification

• Separates compounds based on 3D structure and steric interactions

• Ideal for isolating closely related isomers

Strong Hydrophobic Interaction

• Highly hydrophobic phase

• Effective for lipids and difficult analytes

Tunable Selectivity

• Adjust separation conditions using:

  • Temperature

  • Mobile phase composition

• Enables optimized purification outcomes

Cogent TYPE‑C™ Technology Advantages

Silica Hydride Surface

• Direct Si–C bonding eliminates need for end‑capping

• Reduced secondary interactions

• Improved peak shape and analyte recovery

Faster Equilibration

• Shorter cycle times

• Increased purification throughput

Robust Column Lifetime

• Resistant to hydrolysis

• Stable under high‑load preparative conditions

• Long operational durability

Typical Applications

• Preparative purification of steroidal compounds

• Isolation of structural isomers

• Lipids and hydrophobic analytes

• Peptides, proteins, and oligonucleotides

• Analytical‑to‑prep method scale‑up

• Applications where C18 or C8 selectivity is insufficient

Important Usage Notes

• Requires preparative HPLC system

• Designed for high‑flow, high‑load purification workflows

• Ideal for maximum‑resolution and high‑purity isolation

• Optimize temperature and mobile phase to control selectivity

Made‑to‑Order & Pricing Information

This is a custom, made‑to‑order item.

• Orders cannot be canceled once placed

• Custom items are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification:

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.