Prep HPLC Column, UDC Cholesterol, 4um, 100A. 21.2mm ID x 100mm Length, Preparative. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 69069‑P21‑100 Cogent UDC‑Cholesterol Preparative HPLC Column is designed for high‑capacity purification with enhanced resolution, utilizing 4 µm TYPE‑C™ silica hydride particles modified with a cholesterol‑terminated ligand.
This stationary phase incorporates an 11‑carbon chain directly bonded to silica hydride with a terminal cholesterol group, forming structured interaction regions that act as molecular “slots.” These allow separations based on molecular geometry, steric fit, and shape, not just hydrophobic interaction—making it especially effective for closely related isomers and complex mixtures.
Key advantages:
Direct Si–C bonding (no end‑capping required)
Reduced silanol activity and secondary interactions
Enhanced durability and reproducibility under preparative conditions
Compatible with multiple chromatographic modes:
Reversed Phase
Normal Phase
HILIC
Preparative Column Use‑Case Framework
100 mm Length – Increased Resolution Purification
Higher resolving power than 50 mm columns
Improved fraction purity and separation of closely related compounds
Balanced performance between throughput and resolution
Ideal for scale‑up from analytical methods to prep workflows
Flow Rate & System Requirements (21.2 mm ID)
Preparative columns require specialized HPLC systems
Typical flow rate range: ~20 – 60 mL/min
Optimal starting range: ~20 – 45 mL/min for higher resolution
Requires:
Preparative HPLC system with high‑flow capability
High‑capacity pump heads and tubing
Fraction collection system
⚠️ Standard analytical HPLC systems are not suitable for this column size.
Flow rates should be optimized based on:
Sample load and injection volume
Resolution vs throughput target
Solvent viscosity
System pressure limits
Why Choose UDC‑Cholesterol™ for Preparative Work
Shape‑Selective Purification
Separates compounds based on 3D structure and steric interaction
Excellent for isolating structurally similar analytes and isomers
Strong Hydrophobic Interaction
Highly hydrophobic phase
Effective retention for lipids and difficult compounds
Tunable Selectivity
Adjust separation with:
Temperature changes
Mobile phase composition
Enables optimized purification methods
Cogent TYPE‑C™ Technology Advantages
Silica Hydride Surface
Direct Si–C bonding (no end‑capping required)
Reduced secondary interactions
Improved peak shape and analyte recovery
Faster Equilibration
Shorter cycle times
Increased purification throughput
Robust Column Lifetime
Resistant to hydrolysis
Stable under high‑load preparative conditions
Long operational durability
Typical Applications
Preparative purification of steroidal compounds
Isolation of structural isomers
Lipids and hydrophobic analytes
Peptides, proteins, and oligonucleotides
Analytical‑to‑prep method scale‑up
Applications where C18 or C8 selectivity is insufficient
Important Usage Notes
Requires preparative HPLC system
Designed for high‑flow, high‑load purification workflows
Ideal for balanced purification (resolution + throughput)
Optimize temperature and mobile phase to control selectivity
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable