CUSTOM ITEM: HPLC Column, UDC Cholesterol, 4um, 3.0mm ID x 250mm Length, 100A. Cogent TYPE-C Brand. 1 EA. CUSTOM ORDER.
Additional Info:
The 69069‑25P‑3 Cogent UDC‑Cholesterol HPLC Column is designed for maximum separation and peak capacity, using 4 µm TYPE‑C™ silica hydride particles modified with a cholesterol‑terminated ligand to deliver unique shape‑based selectivity.
This stationary phase incorporates an 11‑carbon linker directly bonded to silica hydride with a terminal cholesterol group, creating structured interaction zones that act as molecular “slots.” These enable analytes to be separated based on molecular geometry and steric fit, not just hydrophobic interaction—providing selectivity beyond conventional reversed‑phase columns.
Key structural advantages:
Direct Si–C bonding (no end‑capping required)
Reduced silanol interactions
Improved reproducibility and robustness
This column supports multiple chromatographic modes:
Reversed Phase
Normal Phase
HILIC
250 mm Length – Maximum Separation & Peak Capacity
Highest resolving power in the 3.0 mm UDC column range
Maximizes peak capacity for highly complex mixtures
Ideal for difficult separations requiring maximum purity
Best suited for method development and final analytical optimization
Longer run times compared to 50 mm, 100 mm, and 150 mm formats
3.0 mm ID – Solvent‑Saving Performance
Comparable chromatographic behavior to 4.6 mm ID columns
Uses significantly less mobile phase
Typical flow rate range: ~0.4–0.6 mL/min
Optimal starting range: ~0.40–0.50 mL/min for highest resolution
Benefits:
Lower solvent consumption and cost
Reduced waste generation
No need for specialized low‑flow systems
✅ Fully compatible with standard HPLC systems
Why Choose UDC‑Cholesterol™
Shape & Isomer Selectivity
Separates analytes based on 3D structure and steric orientation
Ideal for isomers and closely related compounds
Strong Hydrophobic Interaction
Highly hydrophobic stationary phase
Excellent retention of lipids and difficult analytes
Tunable Selectivity
Adjust separation behavior using:
Temperature changes
Mobile phase composition
Enables precise method control
Cogent TYPE‑C™ Technology Advantages
Silica Hydride Surface
Direct Si–C bonding eliminates end‑capping requirement
Reduced silanol activity
Improved peak shape for basic and polar compounds
Faster Equilibration
Faster equilibration than traditional RP and HILIC columns
Reduced run‑to‑run cycle time
Robust Column Lifetime
Resistant to hydrolysis
Stable across a wide range of solvents
Long operational durability
Typical Applications
Steroidal compound analysis
Structural isomer separations
Lipids and hydrophobic compounds
Peptides, proteins, and oligonucleotides
Complex multi‑component mixtures
Analytical workflows requiring maximum separation power
Important Usage Notes
Designed for standard HPLC systems (no special equipment required)
Ideal for reducing solvent use vs 4.6 mm columns
Optimize temperature and solvent composition to fine‑tune selectivity
Best suited for maximum‑resolution separations where shorter columns are insufficient
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable