Catalog Number - 69069-25P

The 69069‑25P Cogent UDC‑Cholesterol HPLC Column is designed for maximum separation performance, utilizing 4 µm TYPE‑C™ silica hydride particles functionalized with a cholesterol‑terminated ligand to deliver unique shape‑based selectivity.

This stationary phase consists of an 11‑carbon chain directly bonded to silica hydride with a terminal cholesterol group, creating structured interaction regions that act as molecular “slots.” These allow compounds to be separated based on molecular geometry, steric orientation, and shape, in addition to hydrophobic interaction—providing selectivity that conventional reversed‑phase columns cannot achieve.

Key structural advantages include:

• Direct Si–C bonding (no end‑capping required)

• Reduced silanol activity and secondary interactions

• Improved reproducibility and long‑term robustness

This column supports multiple chromatographic modes:

• Reversed Phase

• Normal Phase

• HILIC

250 mm Length – Maximum Separation & Retention

• Highest resolving power in the 4.6 mm column range

• Maximizes peak capacity for complex samples

• Ideal for closely related compounds and difficult separations

• Provides longer retention times when needed

• Best suited for final method optimization and high‑resolution analysis

4.6 mm ID – Standard Analytical Performance

• Industry‑standard HPLC format

• Compatible with all conventional HPLC systems

• Typical flow rate range: ~0.8–1.5 mL/min

• Optimal starting range: ~1.0–1.2 mL/min

Benefits:

• Strong detector response

• High reproducibility

• Easy method transfer across systems

Why Choose UDC‑Cholesterol™

Shape & Isomer Selectivity

• Separates compounds based on 3D structure and steric fit

• Ideal for closely related analytes and isomers

Highly Hydrophobic Phase

• Strong retention of lipids and hydrophobic compounds

• Effective for difficult‑to‑retain analytes

Tunable Selectivity

• Separation can be adjusted using:

  • Temperature changes

  • Mobile phase composition

• Enables precise control of difficult separations

Cogent TYPE‑C™ Technology Advantages

Silica Hydride Surface

• Direct Si–C bonding eliminates need for end‑capping

• Reduced silanol interactions

• Improved peak shape for basic and polar compounds

Faster Equilibration

• Faster than traditional reversed‑phase or HILIC columns

• Reduced cycle time between runs

Robust Column Lifetime

• Resistant to hydrolysis

• Stable under a wide range of conditions

• Extended operational durability

Typical Applications

• Steroidal compound analysis

• Structural isomer separations

• Lipids and hydrophobic analytes

• Peptides, proteins, and oligonucleotides

• Complex multi‑component mixtures

• Methods requiring maximum separation and retention

Important Usage Notes

• Ideal for standard HPLC systems (no special equipment required)

• Best suited for maximum‑resolution analytical workflows

• Use temperature and solvent optimization to fine‑tune selectivity

• Selected when shorter columns do not provide sufficient separation