CUSTOM ITEM: HPLC Column, UDC Cholesterol, 4um, 3.0mm ID x 150mm Length, 100A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 69069‑15P‑3 Cogent UDC‑Cholesterol HPLC Column is designed for maximum‑resolution separations while maintaining reduced solvent consumption, using 4 µm TYPE‑C™ silica hydride particles modified with a cholesterol‑terminated ligand.
This stationary phase incorporates an 11‑carbon chain directly bonded to silica hydride with a cholesterol molecule at the terminus, forming structured interaction regions that act as molecular “slots.” These allow compounds to be separated according to molecular shape, steric fit, and geometry, not just hydrophobic interaction—providing selectivity unavailable from traditional reversed‑phase columns.
Key advantages:
Direct Si–C bonding (no end‑capping required)
Reduced silanol interactions
Improved reproducibility and robustness
The column supports multiple chromatographic modes:
Reversed Phase
Normal Phase
HILIC
150 mm Length – Maximum Resolution
Highest resolving power in the 3.0 mm column family
Ideal for complex mixtures and difficult separations
Maximizes isomer separation and peak capacity
Preferred when maximum purity and resolution are required
Longer run times vs 50 mm and 100 mm columns
3.0 mm ID – Solvent‑Saving Performance
Comparable separation performance to 4.6 mm ID columns
Uses significantly less mobile phase
Typical flow rate range: ~0.4–0.6 mL/min
Optimal starting range: ~0.40–0.50 mL/min for higher resolution
Benefits:
Reduced solvent cost and waste
Lower operating expenses
No specialized low‑flow instrumentation required
✅ Fully compatible with standard HPLC systems
Why Choose UDC‑Cholesterol™
Shape & Isomer Selectivity
Separates analytes based on 3D structure and steric orientation
Ideal for isomers and closely related compounds
Strong Hydrophobic Interaction
Highly hydrophobic stationary phase
Excellent retention of lipids and difficult analytes
Tunable Selectivity
Adjust selectivity using:
Temperature changes
Mobile phase composition
Enables precise control for challenging separations
Cogent TYPE‑C™ Technology Advantages
Silica Hydride Surface
Direct Si–C bonding eliminates need for end‑capping
Reduced silanol activity
Improved peak shape for basic and polar compounds
Faster Equilibration
Faster equilibration than traditional RP and HILIC phases
Reduced run‑to‑run cycle time
Robust Column Lifetime
Resistant to hydrolysis
Stable across wide solvent systems
Long operational durability
Typical Applications
Steroidal compound analysis
Structural isomer separations
Lipids and hydrophobic compounds
Peptides, proteins, and oligonucleotides
Analytical methods requiring maximum resolution
Workflows transitioning from 4.6 mm to solvent‑saving formats
Important Usage Notes
Designed for standard HPLC systems (no special equipment required)
Ideal for reducing solvent use vs 4.6 mm columns
Optimize temperature and mobile phase to fine‑tune selectivity
Best suited for complex separations where maximum resolution is required
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable