Catalog Number - 69069-15P-2

The 69069‑15P‑2 Cogent UDC‑Cholesterol HPLC Column is engineered for maximum‑resolution separations, utilizing 4 µm TYPE‑C™ silica hydride particles modified with a cholesterol‑terminated ligand.

This stationary phase incorporates an 11‑carbon chain directly bonded to silica hydride with a terminal cholesterol moiety, creating structured interaction regions that act as molecular “slots.” These enable separation based on molecular geometry, steric orientation, and shape, rather than hydrophobicity alone—providing unique selectivity not achievable with conventional reversed‑phase columns.

Key structural advantages:

• Direct Si–C bonding eliminates need for end‑capping

• Reduced silanol interactions

• Improved reproducibility and robustness

This column supports multiple chromatographic modes:

• Reversed Phase

• Normal Phase

• HILIC

150 mm Length – Maximum Resolution for LC‑MS

• Highest resolving power within the 2.1 mm (4 µm) column range

• Ideal for complex mixtures and difficult separations

• Maximizes isomer separation and peak capacity

• Preferred for LC‑MS workflows requiring highest resolution

• Longer run times compared to 50 mm and 100 mm formats

Flow Rate & System Requirements (2.1 mm ID, 4 µm)

• Typical flow rate range: ~0.2–0.4 mL/min

• Optimal starting range: ~0.18–0.28 mL/min for maximum resolution

• Requires:

  • Reliable low‑flow HPLC or LC‑MS system

  • Low‑volume detector flow cell

  • Stable and precise flow delivery

⚠️ Standard HPLC systems must be capable of consistent low‑flow performance to achieve expected results.

Why Choose UDC‑Cholesterol™

Shape & Isomer Selectivity

• Separates compounds based on 3D structure and steric interactions

• Ideal for isomers and structurally similar analytes

Strong Hydrophobic Interaction

• Highly hydrophobic stationary phase

• Effective retention of lipids and difficult compounds

Tunable Selectivity

• Selectivity controlled by:

  • Temperature adjustments

  • Mobile phase composition

• Enables advanced method optimization

Cogent TYPE‑C™ Technology Advantages

Silica Hydride Surface

• Direct Si–C bonding (no end‑capping required)

• Reduced silanol activity

• Improved peak shape for basic and polar compounds

Faster Equilibration

• Faster than conventional RP and HILIC columns

• Improved throughput despite longer column length

Robust Column Lifetime

• Resistant to hydrolysis

• Stable across varied solvents

• Long operational durability

Typical Applications

• Steroidal compound analysis

• Structural isomer separations

• Lipids and hydrophobic analytes

• Peptides, proteins, and oligonucleotides

• LC‑MS workflows requiring maximum resolution

• Applications where C18 or C8 selectivity is insufficient

Important Usage Notes

• Designed for LC‑MS and low‑flow analytical methods

• Requires precise flow control for best performance

• Use temperature and solvent composition to optimize selectivity

• Best suited for complex separations requiring highest resolution