Catalog Number - 69069-15P

The 69069‑15P Cogent UDC‑Cholesterol HPLC Column is designed for maximum‑resolution separations, delivering unique shape‑based selectivity using 4 µm TYPE‑C™ silica hydride particles modified with a cholesterol‑terminated ligand.

This stationary phase features an 11‑carbon chain directly bonded to silica hydride with a cholesterol group at the terminus, forming structured interaction sites that act as molecular “slots.” These allow analytes to be separated based on molecular shape, steric orientation, and geometry, not just hydrophobicity—enabling separations beyond conventional reversed‑phase columns.

Unlike traditional silica phases:

• Direct Si–C bonding eliminates the need for end‑capping

• Reduced silanol activity and secondary interactions

• Improved reproducibility and long‑term stability

This column supports multiple chromatographic modes:

• Reversed Phase

• Normal Phase

• HILIC

150 mm Length – Maximum Resolution & Peak Capacity

• Highest resolving power in the 4.6 mm analytical column range

• Ideal for complex mixtures and difficult separations

• Maximizes isomer separation and peak capacity

• Preferred for analytical methods requiring highest resolution

• Longer run times compared to 50 mm and 100 mm formats

4.6 mm ID – Standard Analytical Performance

• Industry‑standard format for analytical HPLC

• Compatible with all conventional HPLC systems

• Typical flow rate range: ~0.8–1.5 mL/min

• Optimal starting range: ~1.0–1.2 mL/min

Provides:

• Strong detector response

• Robust reproducibility

• Easy scalability and method transfer

Why Choose UDC‑Cholesterol™

Shape & Isomer Selectivity

• Separates compounds based on 3D molecular structure and steric fit

• Ideal for isomers and closely related analytes

Very Hydrophobic Phase

• Strong retention of lipids and highly hydrophobic compounds

• Effective for difficult‑to‑retain analytes

Tunable Selectivity

• Fine‑tune separations using:

  • Temperature adjustments

  • Mobile phase composition

• Enables flexible method optimization

Cogent TYPE‑C™ Technology Advantages

Silica Hydride Surface

• Direct Si–C bond (no end‑capping required)

• Reduced silanol interactions

• Improved peak shape for basic and polar compounds

Faster Equilibration

• Faster equilibration than traditional RP or HILIC columns

• Reduced run‑to‑run cycle time

Robust Column Lifetime

• Resistant to hydrolysis

• Stable across multiple solvent systems

• Extended operational durability

Typical Applications

• Steroidal compound analysis

• Structural isomer separations

• Lipids and hydrophobic analytes

• Peptides, proteins, and oligonucleotides

• High‑resolution analytical workflows

• Methods where C18/C8 columns lack selectivity

Important Usage Notes

• Ideal for standard HPLC systems (no special instrumentation required)

• Best suited for maximum‑resolution separations

• Optimize temperature and solvent composition to control selectivity

• Common choice for hydrophobic compounds requiring improved separation