CUSTOM ITEM: HPLC Column, UDC Cholesterol, 4um, 3.0mm ID x 100mm Length, 100A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 69069‑10P‑3 Cogent UDC‑Cholesterol HPLC Column provides unique shape‑based selectivity using 4 µm TYPE‑C™ silica hydride particles functionalized with a cholesterol‑terminated ligand.
This stationary phase features an 11‑carbon chain directly bonded to silica hydride with a terminal cholesterol group, forming structured interaction sites that act as molecular “slots.” These allow compounds to be separated based on molecular shape, steric orientation, and geometry, beyond traditional hydrophobic interactions.
Key design advantages:
Direct Si–C bonding (no end‑capping required)
Reduced silanol interactions
Improved reproducibility and robustness
The column supports multiple chromatographic modes:
Reversed Phase
Normal Phase
HILIC
100 mm Length – Increased Resolution
Higher resolving power vs 50 mm columns
Improved isomer separation and peak capacity
Ideal for more complex mixtures and LC‑MS workflows
Balanced run time vs separation efficiency
3.0 mm ID – Solvent‑Saving Alternative
Performance similar to 4.6 mm ID columns
Uses significantly less mobile phase
Typical flow rate range: ~0.4–0.6 mL/min
Optimal starting range: ~0.45–0.55 mL/min
Benefits:
Reduced solvent consumption and cost
Lower waste generation
No special instrumentation required
✅ Fully compatible with standard HPLC systems.
Why Choose UDC‑Cholesterol™
Shape & Isomer Selectivity
Separates compounds by 3D structure and steric fit
Ideal for isomers and structurally similar molecules
Strong Hydrophobic Interaction
Highly hydrophobic stationary phase
Effective for lipids and difficult analytes
Tunable Selectivity
Adjust selectivity using:
Temperature changes
Mobile phase composition
Enables precise method optimization
Cogent TYPE‑C™ Technology Advantages
Silica Hydride Surface
Direct Si–C bond eliminates need for end‑capping
Reduced silanol activity
Improved peak shape for basic and polar compounds
Faster Equilibration
Faster than conventional RP and HILIC phases
Reduced cycle time
Robust Column Lifetime
Resistant to hydrolysis
Stable across wide solvent ranges
Long operational durability
Typical Applications
Steroidal compound analysis
Structural isomer separations
Lipids and hydrophobic analytes
Peptides, proteins, and oligonucleotides
LC‑MS workflows requiring enhanced resolution
Methods transitioning from 4.6 mm to solvent‑saving formats
Important Usage Notes
Designed for standard HPLC systems (no special equipment required)
Ideal for reducing solvent use vs 4.6 mm columns
Optimize temperature and solvent composition to tune selectivity
Best suited for separations where traditional RP phases are insufficient
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable