Catalog Number - 69069-10P-2

The 69069‑10P‑2 Cogent UDC‑Cholesterol HPLC Column delivers unique shape‑based selectivity using 4 µm TYPE‑C™ silica hydride particles modified with a cholesterol‑terminated ligand.

This stationary phase consists of an 11‑carbon chain directly bonded to silica hydride with a cholesterol moiety at the terminus, forming structured interaction regions that act as molecular “slots.” These allow compounds to be separated based on molecular geometry and steric fit, not just hydrophobicity—enabling separations that conventional reversed‑phase columns cannot achieve.

Unlike traditional silica phases:

• Direct Si–C bonding eliminates the need for end‑capping

• Reduced silanol interactions

• Improved reproducibility and long‑term stability

This column supports multiple chromatographic modes:

• Reversed Phase

• Normal Phase

• HILIC

100 mm Length – Increased Resolution for LC‑MS

• Higher resolving power vs 50 mm columns

• Improved isomer separation and peak capacity

• Ideal for LC‑MS workflows requiring enhanced resolution

• Balanced performance between speed and separation quality

This is one of the most popular formats for 2.1 mm analytical LC‑MS methods, where better separation is required without excessive run time.

Flow Rate & System Requirements (2.1 mm ID, 4 µm)

• Typical flow rate range: ~0.2–0.4 mL/min

• Optimal starting range: ~0.2–0.3 mL/min

• Requires:

  • Reliable low‑flow HPLC or LC‑MS system

  • Low‑volume detector flow cell

  • Precision pumping for stable flow delivery

⚠️ Standard HPLC systems are acceptable only if capable of stable low‑flow performance.

Why Choose UDC‑Cholesterol™

Shape & Isomer Selectivity

• Separates analytes based on 3D structure and steric orientation

• Ideal for isomers and structurally similar compounds

Strong Hydrophobic Retention

• Highly hydrophobic stationary phase

• Effective retention for lipids and difficult compounds

Tunable Selectivity

• Selectivity can be controlled through:

  • Temperature changes

  • Mobile phase composition

• Enables flexible method development

Cogent TYPE‑C™ Technology Advantages

Silica Hydride Surface

• Direct Si–C bonding (no end‑capping required)

• Reduced silanol activity

• Improved peak shape for basic and polar compounds

Faster Equilibration

• Faster equilibration than conventional RP or HILIC columns

• Improved throughput

Robust Column Lifetime

• Resistant to hydrolysis

• Stable across a wide range of solvents and conditions

• Long operational durability

Typical Applications

• Steroidal compound analysis

• Structural isomer separations

• Lipids and hydrophobic analytes

• Peptides, proteins, and oligonucleotides

• LC‑MS workflows requiring increased resolution

• Compounds poorly resolved on C18 or C8 phases

Important Usage Notes

• Optimized for LC‑MS and low‑flow analytical methods

• Requires precise flow control for best performance

• Use temperature and mobile phase to fine‑tune selectivity

• Ideal when traditional reversed‑phase selectivity is insufficient