Catalog Number - 69069-05P-3

The 69069‑05P‑3 Cogent UDC‑Cholesterol HPLC Column delivers unique shape‑based selectivity using 4 µm TYPE‑C™ silica hydride particles modified with a cholesterol‑terminated ligand.

This stationary phase consists of an 11‑carbon chain directly bonded to silica hydride with a cholesterol group at the terminus, forming structured interaction regions that act as molecular “slots.” These enable separation based on molecular shape and steric fit, not just hydrophobicity—providing selectivity beyond traditional reversed‑phase columns.

Key structural advantages:

• Direct Si–C bonding (no end‑capping required)

• Reduced silanol interactions

• Improved reproducibility and robustness

This column supports multiple chromatographic modes:

• Reversed Phase

• Normal Phase

• HILIC

50 mm Length – Speed & Throughput

• Optimized for fast separations and short run times

• Lower backpressure compared to longer columns

• Ideal for screening and rapid method development

• Suitable for high‑throughput analytical workflows

3.0 mm ID – Solvent‑Saving Performance

• Provides similar chromatography to 4.6 mm ID columns

• Uses significantly less mobile phase

• Operates at lower flow rates (~0.4–0.6 mL/min typical)

• Ideal for reducing solvent consumption and operating costs

✅ Compatible with standard HPLC systems (no special low‑flow hardware required).

Why Choose UDC‑Cholesterol™

Shape & Isomer Selectivity

• Separates compounds based on 3D structure and steric orientation

• Excellent for isomers and structurally similar analytes

Strong Hydrophobic Interaction

• Highly hydrophobic phase

• Effective retention of lipids and difficult compounds

Tunable Selectivity

• Selectivity can be adjusted using:

  • Temperature changes

  • Mobile phase composition

• Enables advanced method optimization

Cogent TYPE‑C™ Technology Advantages

Silica Hydride Surface

• Direct Si–C bonding eliminates need for end‑capping

• Reduced silanol activity

• Improved peak shape for basic and polar compounds

Faster Equilibration

• Rapid equilibration vs traditional RP and HILIC columns

• Increased throughput

Robust Column Lifetime

• Resistant to hydrolysis

• Stable across varied solvents and conditions

• Long operational durability

Typical Applications

• Steroidal compound analysis

• Lipids and hydrophobic molecules

• Peptides, proteins, and oligonucleotides

• Structural isomer separations

• Analytical workflows requiring alternative selectivity

• Methods transitioning from 4.6 mm to solvent‑saving formats

Important Usage Notes

• Designed for standard HPLC systems (no special equipment required)

• Ideal for reducing solvent consumption vs 4.6 mm columns

• Optimize temperature and mobile phase to fine‑tune selectivity

• Excellent option when C18/C8 phases cannot resolve isomers

Made‑to‑Order & Pricing Information

This is a custom, made‑to‑order item.

• Orders cannot be canceled once placed

• Custom items are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification:

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.