Catalog Number - 40218-05P-2

The 40218‑05P‑2 Cogent Bidentate C18™ 2.o HPLC Column is a high‑efficiency, narrow‑bore reversed‑phase column engineered for laboratories seeking near‑UHPLC performance on standard HPLC instruments. Built on Cogent TYPE‑C™ silica hydride technology, this column combines smaller 2.2 µm particles with bidentate C18 bonding to deliver increased efficiency, excellent selectivity, and fast equilibration—without requiring UHPLC‑rated hardware.

The silica hydride surface with direct Si–C bonding produces exceptional chemical robustness and reduced secondary interactions relative to conventional silica‑based C18 columns. The Bidentate C18™ 2.o chemistry is particularly effective for very hydrophobic and non‑polar compounds that are difficult to retain under aqueous mobile‑phase conditions, while remaining mass‑spectrometry friendly.

The 2.1 mm ID × 50 mm geometry supports low‑flow operation, enhancing MS sensitivity and reducing solvent consumption. Because of the reduced internal volume and higher efficiency, this column should be used with pumps capable of precise low‑flow delivery and detector flow cells designed for reduced volumes.

Why Bidentate C18™ 2.o Is Different

While conventional C18 columns rely on siloxane bonding that can degrade at extremes of pH or lose retention in highly aqueous systems, the Cogent Bidentate C18™ 2.o offers:

• Silica hydride surface chemistry with low silanol activity

• Direct Si–C bonds for superior chemical stability

• Near‑UHPLC efficiency using 2.2 µm particles on standard HPLC systems

• Excellent retention in aqueous‑dominant mobile phases

• Robust operation at low pH without column damage

This makes it ideal for demanding reversed‑phase and LC‑MS methods requiring speed, sensitivity, and robustness.

Key Features

Cogent TYPE‑C™ Silica Hydride Technology

• Reduced silanol interactions and faster equilibration

• Exceptional stability and long column lifetime

• Consistent performance across aggressive conditions

Bidentate C18™ 2.o Bonding

• Two‑point attachment for enhanced durability

• High hydrophobicity with versatile selectivity

• Retains very non‑polar compounds under aqueous conditions

Near‑UHPLC Efficiency (2.2 µm)

• Increased efficiency vs. conventional 3–5 µm columns

• High performance on standard HPLC instruments

• Faster separations and improved peak capacity

LC‑MS Optimized Geometry

• 2.1 mm ID enhances sensitivity and reduces solvent use

• Ideal for ESI‑MS and LC‑MS/MS workflows

• Short 50 mm length enables rapid analysis

System Requirements

Due to the small particle size and narrow‑bore format, optimal performance requires:

• A pump capable of precise, stable low‑flow delivery

• Reduced‑volume flow cells suitable for 2.1 mm ID columns

Typical Applications

• LC‑MS and LC‑MS/MS

• High‑efficiency reversed‑phase separations

• Hydrophobic and highly non‑polar compounds

• Aqueous‑dominant mobile phases

• Fast method development and high‑throughput analysis