3,3′-Diaminobenzidine by LCMS – AppNote

Retention and Separation is easy with the Cogent Diamond Hydride Column with the Method below. Normally this compound is very hard to get good peak shapes in Reversed Phase. Peak: 3,3′-Diaminobenzidine 215.1291 m/z (M+H)+ Method Conditions: Column: Cogent Diamond Hydride™, 4µm, 100Å Catalog No.: 70000-15P-2 Dimensions: 2.1 x 150 mm Solvents: A: 50% DI Water/ […]

Read More

Bisphenol A, S, & F Separated by HPLC – AppNote

Separation of Epoxy Resins Found in Consumer Products & Food Many consumer products are using BPA alternatives in recent years due to health concerns regarding toxicity of these compounds.  An analytical HPLC method is shown in this AppNote to distinguish amongst these various Bisphenol compounds potentially in chemicals and foods. Excellent resolution was obtained for […]

Read More

EDTA Analysis by HPLC – AppNote

EDTA does not have a significant Chromophore, so to achieve UV Detection, in the Method shown below we used a pre-Column reaction of a Solution of Ferric Chloride with the Sample. The resulting EDTA/Fe3+ has significant UV absorbance making this a very Sensitive Method. Ethylenediaminetetraacetic acid is extremely difficult to analyze by itself however in […]

Read More

Herbicide and Metabolites by LCMS – AppNote

Glufosinate, N-Acetylglufosinate, and Glufosinate Propanoic Acid Analysis of these Compounds can be problematic with other methods and poor peak shape may occur. In contrast, the Peaks obtained in this method are very sharp and symmetrical and can be applied to food products containing these types of Compounds. Peaks: 1. Glufosinate m/z 180.0431 [M-H]– 2. N-Acetylglufosinate […]

Read More

Herbicides & Metabolite Analysis by LCMS- AppNote

Glufosinate, N-Acetylglufosinate, Glufosinate Propanoic Acid, Diquat  & Paraquat Two primary metabolites of Glufosinate are N-Acetylglufosinate and Glufosinate Propanoic Acid. Due to the potentially toxic nature of these Herbicides, analysis of their metabolites in a variety of sample matrices may be required. However, all three compounds are quite polar and therefore difficult to retain with conventional […]

Read More

Histamine Content in Red Wine by LCMS – AppNote

Demonstrating Low Histamine Content in Red Wine Samples Two red wine samples were tested for Histamine using the Cogent Diamond Hydride Column in an LC-MS method. The wine shown in Figure B was claimed to have virtually no Histamine but had no analytical evidence to support it. The claim was verified using this simple LCMS […]

Read More

Metaldehyde in Slug Pellets Analyzed by LCMS – AppNote

Pesticide Formulation As Metaldehyde is a non-UV absorbing compound, other detection methods needed to be investigated besides conventional UV-HPLC. LC-MS was found to be a well-suited analysis by searching for the EIC corresponding to the [M+H]+ ion. Good retention and peak shape were observed for this analyte using the Cogent Bidentate C18 2.o™ Column. Peak: […]

Read More

Morphine Sulfate Tablet HPLC Method Transferred to Near UHPLC – AppNote

Standard 4um Particle Size Transferred to 2.o™ This application illustrates how methods developed using the 4µm Cogent Diamond Hydride Columns may be adapted for Cogent Diamond Hydride 2.o™ phases. Morphine shows slightly higher retention on the 2.o™ Column (average 7.311 min vs. 5.588 min). The efficiency is almost twice as high when using the smaller […]

Read More

Organic Alcohols Separated by HPLC – AppNote

Separation of the Organic Alcohols, 1-Phenyl-2-propanol and 2-Phenyl-1,2-propanediol by HPLC in 5 different lots to show excellent precision and very low RSD. Organic Alcohols Method Conditions: Column: Cogent Bidentate C18™, 4µm, 100Å Catalog No.: 40018-75P Dimensions: 4.6 x 75 mm Solvents: A: DI H20 / 0 .1% Formic Acid (v/v) B: Acetonitrile/ 0.1% Formic Acid […]

Read More

Organic Bases and Isomers at a Low pH – AppNote

Separation of Four Amine Containing Test Solutes The four test solutes selected for this application are both well-retained and well-resolved. In particular, the separation between the two isomers is readily accomplished, which may be difficult to achieve in Reversed Phase. This Method uses only Formic Acid as the Mobile Phase additive and is LCMS compatible. […]

Read More

Pyrilamine & 4-Amino-3-Chloropyridine – AppNote

Unique Selectivity on an Amide Stationary Phase The Cogent Amide Column offers unique selectivity that may not be readily attainable with other phases. Two test solutes shown in this application note (Pyrilamine and 4-Amino-3-Chloropyridine) were baseline separated on the Cogent Amide Column (Figure A), but  they co-eluted with no resolution on a different Cogent Column […]

Read More

Separation of Polar Solutes by HPLC – AppNote

Niacin, Riboflavin, Folic Acid, Pyridoxine, Metformin, Thiamine The Cogent Diol Column is a good addition to the TYPE-C™ Silica line of HPLC stationary phases. Here, a variety of common polar analytes are well-retained and separated. Peaks: 1. Ascorbic acid 2. Niacin 3. Riboflavin 4. Folic acid 5. Pyridoxine 6. Metformin 7. Thiamine Method Conditions Column: […]

Read More

Tetrahydrocannabinol (THC) – AppNote

Psychoactive Component in Cannabis  Tetrahydrocannabinol has several isomeric forms, which may account for the extra peaks observed in the Chromatogram below. Although their identities could not be confirmed, these peaks can be separated from the main peak using the Cogent Bidentate C18  Column. The compound is quite hydrophobic and therefore a relatively high organic content […]

Read More

Transfer HPLC Method to UHPLC with Hydrophobic Compounds – AppNote

Separation of Hydrophobic Compounds by HPLC & UHPLC This AppNote shows separation of analytes within a range of hydrophobicity. A simple gradient is used to elute all the compounds and baseline separation is obtained for the critical pair (peaks 4 and 5) and the least hydrophobic compound is adequately retained. A comparison is shown in […]

Read More

Zinc-EDTA Complex – AppNote

No Ion Pair Agents Needed Using conventional analytical methods, retention of metal–EDTA complexes is accomplished using Ion Pair Reversed Phase Chromatography. However, the Ion Pair Agents used in the Mobile Phase are not compatible with Mass Spectrometry. In this AppNote, only Formic Acid is needed in the Mobile Phase in order to obtain retention of […]

Read More