Fluoxetine (Prozac®) – AppNote

Excellent Retention and Peak Shape with Amide Column Fluoxetine can have a tendency to tail in some HPLC methods due to its secondary amine group. However, peak shape with the Cogent Amide Column was found to be highly symmetrical without the aid of ion pairing agents. This allows the method conditions to be adapted to […]

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Fructose and Glucose in Coca-Cola Analyzed by HPLC with RI – AppNote

Separation of Polar Sugars in Coca Cola© The high sugar content of soda beverages can be observed in this Chromatographic separation of Fructose and Glucose in cola. The two Sugars are retained and separated with the use of the Cogent Amide Column. Data from reference standards is also shown for peak identity confirmation. A preconditioning […]

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Galactose and Sucrose – AppNote

Retention and Separation of Mono and Disaccharides This application demonstrates the ability of the Cogent Amide Column to retain two highly polar test solutes. Sucrose, for example, has a log P of -3.7 and hence would be unlikely to retain in Reversed Phase. Excellent separation is obtained here for these Mono and Disaccharides. The noisy […]

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Pyrilamine & 4-Amino-3-Chloropyridine – AppNote

Unique Selectivity on an Amide Stationary Phase The Cogent Amide Column offers unique selectivity that may not be readily attainable with other phases. Two test solutes shown in this application note (Pyrilamine and 4-Amino-3-Chloropyridine) were baseline separated on the Cogent Amide Column (Figure A), but  they co-eluted with no resolution on a different Cogent Column […]

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Ribose and Xylose – AppNote

Sugars can be difficult to analyze by HPLC due to their polarity. Columns with amine ligands are often used for retention of simple sugars like Ribose and Xylose, but they have a number of drawbacks. The amine group can form Schiff bases with Aldehydes in the sample, resulting in irreversible deactivation of the ligand’s retention […]

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Tizanidine HCl Tablet – AppNote

Excellent Run to Run Precision Tizanidine has several amine groups which can present problems to chromatographers. Residual silanols on the surface of ordinary Silica-based Columns can electrostatically interact with these groups, causing peak tailing. The Cogent Amide Column, used in the Application Note is virtually free of residual silanols and therefore a symmetrical peak shape […]

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