Although Prep Chromatography or Preparative Chromatography is not simple and has many different aspects and uses and therefore is really specific for each need, there are some generalities that should be considered if you are thinking of working with HPLC for purifications. A higher surface area generally leads to more load-ability. But this is also […]
At the semi-prep or prep scale, other manufacturers tend to use larger particles (about 10–20um/ spherical for HPLC or 40–60um/ irregular for flash) whereas the Cogent™ columns can use 4um spherical particles. Larger particles are cheaper but the Cogent™ columns can save money in other ways. For custom orders, larger particles may be available for Cogent columns. In […]
In our standard 4um material, currently the largest ID we pack is 30mm. This is threaded hardware whereas larger IDs such as 50mm generally must be flanged or bolted. You may want to consider the 21.2mm ID as well, since cost increases at larger diameters. When logged in on the website, you can view the […]
What is the maximum loading capacity (mass, injection volume) that can be used for a “semi-prep” Cogent TYPE-C™ HPLC column?
There is no simple answer to this question as it is matrix, eluent, column phase, and target dependent. Here are some things to consider: 1. Peak Separation. You may be able to get 150–250 mg loading under good conditions, but it may drop to 50 mg or less if you have closely eluting peaks. The reason for […]